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Merck
CN

D4139

N,N-Dimethyl-p-phenylenediamine dihydrochloride

≥99.0% purity (titration), powder

Synonym(s):

4-Amino-N,N-dimethylaniline dihydrochloride, DMPD · 2HCl, DMPPDA · 2HCl

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About This Item

Linear Formula:
(CH3)2NC6H4NH2 · 2HCl
CAS Number:
536-46-9
Molecular Weight:
209.12
NACRES:
NA.47
PubChem Substance ID:
24893818
UNSPSC Code:
12171500
EC Number:
208-635-7
MDL number:
MFCD00012991
Beilstein/REAXYS Number:
3913463

Key Documents

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Product Name

N,N-Dimethyl-p-phenylenediamine dihydrochloride, suitable for peroxidase test, ≥99.0% (titration)

InChI

1S/C8H12N2.2ClH/c1-10(2)8-5-3-7(9)4-6-8;;/h3-6H,9H2,1-2H3;2*1H

InChI key

IAEDWDXMFDKWFU-UHFFFAOYSA-N

SMILES string

Cl[H].Cl[H].CN(C)c1ccc(N)cc1

assay

≥99.0% (titration)

form

powder

technique(s)

titration: suitable

color

white to light pink, and tan

mp

222 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear

suitability

suitable for peroxidase test

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples.

Biochem/physiol Actions

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.

Disclaimer

Darkens readily when exposed to air.

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9588
BCCN0512
BCCL3773
BCCK3221
BCCM5534

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Colorimetric analysis with N, N-dimethyl-p-phenylenediamine of the uptake of intravenously injected horseradish peroxidase by various tissues of the rat.
STRAUS W
The Journal of Biophysical and Biochemical Cytology, 4, 541-541 (1958)
Digestive Disease Pathology (2013)
The distribution of mucous secreting cells in the gastrointestinal tracts of three small rodents from Saudi Arabia: Acomys dimidiatus, Meriones rex and Meriones libycus.
Johnson O, et al.
Acta Histochemica, 118, 118-118 (2016)
A method for serial tissue processing and parallel analysis of aberrant crypt morphology, mucin depletion, and Beta-catenin staining in an experimental model of colon carcinogenesis.
McGinley JN, et al.
Biological Procedures Online, 12, 9032-9032 (2010)
F Tessier et al.
International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition, 66(2), 166-170 (1996-01-01)
An HPLC micro-method with fluorescence detection has been developed to determine total vitamin C (vit C) and dehydroascorbic acid (DHA) concentrations in human plasma samples. This method is based on the rapid, specific reaction of DHA with dimethyl-o-phenylenediamine (DMPD) to
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