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D4168

SIGMAFAST™ 3,3′-Diaminobenzidine tablets

SIGMAFAST™, tablet

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About This Item

CAS Number:
91-95-2
UNSPSC Code:
12352204
PubChem Substance ID:
24893827
NACRES:
NA.83
MDL number:
MFCD00007725

Key Documents

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Product Name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 1 mL

Quality Level

200

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

form

tablet

solubility

deionized water: 1 mL, clear, pink

storage temp.

−20°C

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Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Preparation Note

Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

signalword

Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

5.1B - Oxidizing hazardous materials

flash_point_f

53.6 °F - closed cup

flash_point_c

12 °C - closed cup

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
SLBV8376
SLBP9645V
SLBM4338V
SLBG6151V
SLBG6151

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American journal of physiology. Cell physiology, 312(1), C16-C28 (2016-10-28)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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