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Sigma-Aldrich

SIGMAFAST™ 3,3′-Diaminobenzidine tablets

tablet, To prepare 5 mL

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Synonym(s):
DAB tablets
CAS Number:
91-95-2
MDL number:
MFCD00007725
PubChem Substance ID:
24893856
NACRES:
NA.83

Quality Level

200

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Application

SIGMAFAST™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Interstitial cells of Cajal are present in human extrahepatic bile ducts
Ahmadi O, et al.
Journal of Gastroenterology and Hepatology, 25(2), 277-285 (2010)
Lixing W Reneker et al.
Investigative ophthalmology & visual science, 58(5), 2638-2646 (2017-05-17)
Little is known about the signaling mechanisms controlling meibomian gland (MG) homeostasis and the pathogenic processes leading to MG atrophy and dysfunction in dry eye disease (DED). We investigated the role of fibroblast growth factor receptor 2 (FGFR2) in the
Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis
Salakou S, et al.
In Vivo, 21(1), 123-132 (2007)
Receptor activator NFkappaB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas
Menezes R, et al.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(3), 404-409 (2006)
Expression of pluripotent stem cell markers in the human fetal testis
Kerr CL, et al.
Stem Cells, 26(2), 412-421 (2008)
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