D4293

3,3′-Diaminobenzidine

Name: 3,3′-Diaminobenzidine
Brand: SIGMA

SIGMAFAST^™, chromogenic, tablet

Synonym(s):

DAB tablets

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About This Item

CAS Number:

91-95-2

MDL number:

MFCD00007725

UNSPSC Code:

12352204

PubChem Substance ID:

24893856

NACRES:

NA.83

Key Documents

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Product Name

SIGMAFAST^™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Related Categories

Detection Substrates & Enzymes

Protein Biology

Proteins & Enzymes

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST^™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

SIGMAFAST^™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.

Preparation Note

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Pictograms

GHS08,GHS05,GHS07

Signal Word

Danger

Hazard Statements

H315,H318,H335,H341,H350

Precautionary Statements

P202 - P261 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis

Salakou S, et al.

In Vivo, 21(1), 123-132 (2007)

Receptor activator NFkappaB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas

Menezes R, et al.

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(3), 404-409 (2006)

Expression of pluripotent stem cell markers in the human fetal testis

Kerr CL, et al.

Stem Cells, 26(2), 412-421 (2008)

Interstitial cells of Cajal are present in human extrahepatic bile ducts

Ahmadi O, et al.

Journal of Gastroenterology and Hepatology, 25(2), 277-285 (2010)

Single Inflammatory Trigger Leads to Neuroinflammation in LRRK2 Rodent Model without Degeneration of Dopaminergic Neurons.

Anna Schildt et al.

Journal of Parkinson's disease, 9(1), 121-139 (2018-11-20)

Leucine-rich repeat kinase 2 (LRRK2) mutations are the most common genetic risk factor for Parkinson's disease (PD). While the corresponding pathogenic mechanisms remain largely unknown, LRRK2 has been implicated in the immune system. To assess whether LRRK2 mutations alter the

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比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

3,3′-Diaminobenzidine