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Merck
CN

D4668

Dextranase from Penicillium sp.

lyophilized powder, 100-250 units/mg protein

Synonym(s):

1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-803-9
MDL number:
EC Number:
Specific activity:
100-250 units/mg protein
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form

lyophilized powder

specific activity

100-250 units/mg protein

composition

Protein, ~25% Lowry

storage temp.

2-8°C

Quality Level

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Application

Dextranase from Penicillium sp. can be used to modify alternan. Dextranase from Penicillium sp. has also been used in a study that explored the possibility that the Dex protein might adopt a fold unfamiliar to any known structure.

Biochem/physiol Actions

The product is an endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Physical form

Partially purified, lyophilized powder

Other Notes

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Timothy D Leathers et al.
Biotechnology letters, 31(2), 289-293 (2008-10-22)
Alternan is a unique glucan with a backbone structure of alternating alpha-(1 --> 6) and alpha-(1 --> 3) linkages. Previously, we isolated strains of Penicillium sp. that modify native, high molecular weight alternan in a novel bioconversion process to a
T Pons et al.
Proteins, 31(4), 345-354 (1998-06-17)
The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small isomaltosaccharides. Sequence analysis has revealed only one homologous sequence, CB-8 protein, from Arthrobacter sp., with 30% sequence identity.
Dextranase in sugar industry: A review
Efrain Rodriguez Jimenez
Sugar Tech, 11(2), 124-134 (2009)
Hongbin Zhang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(4), 495-503 (2011-07-30)
To obtain new fungi producing dextranase,we screened and identified a strain F1001 showing high dextranase activities. We provided a new strain with dextranase activity for producing clinical dextran. Morphological and ITS rDNA sequences homology analysis were performed to identify the
Young-Min Kim et al.
Applied microbiology and biotechnology, 91(2), 329-339 (2011-04-12)
Multiple forms of native and recombinant endo-dextranases (Dexs) of the glycoside hydrolase family (GH) 66 exist. The GH 66 Dex gene from Streptococcus mutans ATCC 25175 (SmDex) was expressed in Escherichia coli. The recombinant full-size (95.4 kDa) SmDex protein was digested

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