D4889
Deoxyribonucleic acid sodium salt from Escherichia coli strain B
Genomic, unsheared
Synonym(s):
DNA
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
biological source
Escherichia coli
Quality Level
grade
Molecular Biology
form
lyophilized powder
mol wt
>45 kb
solubility
water: ~1 g/L
storage temp.
2-8°C
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General description
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.
Application
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
- PCR reactions of genomic DNA of Gardnerella vaginalis
- nick translation and radiolabeling of dNTP
- generating standard curve for 16S rDNA quantification
- transfecting neutrophils
- testing the extraction methods
Biochem/physiol Actions
50% GC; Tm = 90.5 °C
Preparation Note
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Analysis Note
Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Benito J Regueiro et al.
PloS one, 5(10), e13387-e13387 (2010-10-23)
Sepsis is one of the leading causes of morbidity and mortality in hospitalized patients worldwide. Molecular technologies for rapid detection of microorganisms in patients with sepsis have only recently become available. LightCycler SeptiFast test M(grade) (Roche Diagnostics GmbH) is a
M Agarwal et al.
BJU international, 88(9), 868-870 (2002-02-20)
To investigate the possible role of Gardnerella vaginalis in interstitial cystitis (IC), using molecular methods to avoid difficulties with the culture and recovery of viable organisms, and the problems associated with the recovery of low numbers of culturable organisms. Thirty-three
Yue Huo et al.
Current microbiology, 76(1), 22-28 (2018-11-02)
A Gram-positive bacterium (DCY118T) was isolated from ginseng-cultivated soil in Gochang-gun, Republic of Korea. This isolate was assigned to the genus Ornithinimicrobium and is closely related to Ornithinimicrobium kibberense K22-20T (98.8%), O. pekingense DSM 21552T (98.5%), O. algicola JC311T (98.2%)
Joseph W Duris et al.
Water research, 47(1), 300-314 (2012-11-15)
The occurrence and distribution of fecal indicator bacteria (FIB) and bacterial and protozoan pathogens are controlled by diverse factors. To investigate these factors in Pennsylvania streams, 217 samples were collected quarterly from a 27-station water-quality monitoring network from July 2007
Rudolph Spangler et al.
PloS one, 4(9), e7010-e7010 (2009-09-16)
PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most
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