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D5546

DMEM - low glucose

Name: DMEM - low glucose
Brand: SIGMA

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217342

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Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Dulbecco's Modified Eagle Medium (DMEM)

Molecular Biology & Functional Genomics

General description

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:

human osteosarcoma cells and HeLa cells
primary human mesenchymal stromal cells (hBMSCs)
human embryonic kidney cell lines

Preparation Note

Supplement with 0.584 g/L glutamine.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
D5546-500ML	04061833586440
D5546-6X500ML	04061833586464
D5546-24X500ML	04061833586433
D5546-1L	04061833586426
D5546-6X1L	04061833586457

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