D5884
Dextranase from Penicillium sp.
lyophilized powder, 10-25 units/mg solid
Synonym(s):
1,6-α-D-Glucan 6-glucanohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-803-9
MDL number:
Specific activity:
10-25 units/mg solid
Application
Dextranase from Sigma has been used for the hydrolysis of carbohydate polymers, during the study of polysaccharide synthesis by Phanerochaete chrysosporium. It has also been used in the synthesis of new enzymatically degradable thermo-responsive nanogels.
Dextranase from Penicillium sp. has been used in a study to assess a new method of synthesizing biopolymeric affinity ligands. Dextranase from Penicillium sp. Has also been used in a study to investigate the cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum.
Biochem/physiol Actions
An endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.
Analysis Note
Crude
Other Notes
One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Related Content
B Garcia et al.
FEMS microbiology letters, 143(2-3), 175-183 (1996-10-01)
A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of selected clones
Polysaccharide synthesis by Phanerochaete chrysosporium during degradation of kraft lignin
Leisola M, et al.
Europ. J. Appl. Microbiol. Biotechnol., 15(3), 180-184 (1982)
Synthesis of new enzymatically degradable thermo-responsive nanogels.
Aguirre, G., Ramos, J., & Forcada, J
Soft Matter, 9(1), 261-270 (2013)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D5884-5KU | 04061832843445 |