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D5921

DMEM - low glucose

Name: DMEM - low glucose
Brand: SIGMA

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217342

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Product Name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Classical Media & Buffers

Dulbecco's Modified Eagle Medium (DMEM)

Molecular Biology & Functional Genomics

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:

living colon cancer SW620 cells
human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
mammal and canine derived adipose-derived mesenchymal stem cells

Preparation Note

Supplement with 0.584 g/L L-glutamine.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Fabian E Ortega et al.

eLife, 8 (2019-02-06)

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Treatment with 2-methoxyestradiol increases endothelial nitric oxide synthase activity via scavenger receptor class BI in human umbilical vein endothelial cells.

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Molecular human reproduction, 26(6), 441-451 (2020-04-26)

Concentrations of 2-methoxyestradiol (2ME2), a principal metabolite of estradiol, are significantly lower in women with severe preeclampsia. Nitric oxide (NO) released by endothelial nitric oxide synthase (eNOS) plays an important role in regulating cardiovascular homeostasis. Importantly, high-density lipoprotein (HDL) stimulates

Biomechanics of subcellular structures by non-invasive Brillouin microscopy.

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Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and

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Christina Schmid et al.

Journal of pharmacological and toxicological methods, 106, 106915-106915 (2020-09-02)

Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are being evaluated for their use in pharmacological and toxicological testing, particularly for electrophysiological side effects. However, little is known about the composition of the commercially available iCell cardiomyocyte (Fuijifilm Cellular Dynamics) cultures

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Global Trade Item Number

SKU	GTIN
D5921-500ML	04061833587065
D5921-6X500ML	04061833587072

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