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D6444

Anti-DBP5 (N-terminal) antibody produced in rabbit

Name: Anti-DBP5 (N-terminal) antibody produced in rabbit
Brand: SIGMA

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DDX19B, Anti-DEAD (Asp-Glu-Ala-As) box polypetide 19B, Anti-DEAD5, Anti-DED box protein 19B

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About This Item

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

rat (predicted), human, mouse (predicted)

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μL using HEK-293T cell lysate, indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde-fixed HEK-293T cells over-expressing human DBP5, western blot: 1-2 μg/mL using HEK-293T cell lysate

UniProt accession no.

Q9UMR2

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DDX19B(11269)
mouse ... Ddx19b(234733)

Description

General description

DBP5 belongs to the family of the DEAD-box helicases that are involved in cellular RNA metabolism from transcription through pre-mRNA splicing, nuclear export, translation initiation to RNA degradation. It localizes within the cytoplasm and at the nuclear rim, where it interacts with components of the nuclear pore complex (NPC). It shuttles between the nucleus and the cytoplasm by using Nup159 as a binding platform.

Application

Anti-DBP5 antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μL using HEK-293T cell lysate, immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cell lysate and immunofluorescence at a working concentration of 2.5-5μg/mL using paraformaldehyde fixed HEK-293T cells over-expressing human DBP5.

Biochem/physiol Actions

DBP5 is required for mRNA export from the nucleus in an ATP-dependent manner. It is also involved in translation termination, where it recognizes stop-codon by controlling eRF1-eRF3 interaction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)