D7304
3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride
Synonym(s):
Chromogenic substrate, DAB substrate solution
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
form
liquid
shipped in
wet ice
storage temp.
2-8°C
General description
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme and is useful as a staining agent in immunohistological and immunoblotting techniques. The end product is resistant to alcohol therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate System. The DAB Liquid Substrate System provides all the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution..
Application
3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride has been used as a substrate:
- in the immunoblot assay carried out to identify the changes in heat shock proteins (Hsps) expressed in the liver
- for visualization of sections after immunostaining
- in immunohistochemistry of mice knee joint sections
- in the immunolabeling of kisspeptin neurons in the preoptic area of camel
Preparation Note
Each DAB Liquid Substrate System will provide 250 mL of DAB substrate solution.
Disclaimer
Stable at least one year at 2-8 °C.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit Components Only
Product No.
Description
- 10X DAB Liquid Chromogen 25 mL
- ready-to-use buffer/peroxide solution 250 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Regulatory Information
危险化学品
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Ainani H, et al.
The Journal of Comparative Neurology, 528(1), 36-51 (2020)
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J Balthazart et al.
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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