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D8764

Sigma-Aldrich

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine spleen

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

38 kDa

concentration

≥50% protein

technique(s)

DNA extraction: suitable
tissue culture: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

foreign activity

RNase ≤0.1%

storage temp.

−20°C

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Application

Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples
  • to digest retinal explants for single-cell suspension preparation
  • for isolation of stromal cells from human colon tissue
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Unit Definition

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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