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D8764

Deoxyribonuclease II from bovine spleen

Name: Deoxyribonuclease II from bovine spleen
Brand: SIGMA

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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About This Item

CAS Number:

9025-64-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-801-8

MDL number:

MFCD00130919

EC Number:

3.1.22.1 (BRENDA, IUBMB)

Specific activity:

≥1,000 units/mg protein

Biological source:

bovine spleen

Concentration:

≥50% protein

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Properties

biological source

bovine spleen

Quality Segment

200

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

38 kDa

concentration

≥50% protein

technique(s)

DNA extraction: suitable, tissue culture: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

foreign activity

RNase ≤0.1%

storage temp.

−20°C

Description

Application

Deoxyribonuclease II from bovine spleen has been used:

as a component of digestion mix to isolate immune cells from colon samples
to digest retinal explants for single-cell suspension preparation
for isolation of stromal cells from human colon tissue
in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Other Notes

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg²⁺] = 0.83 mM

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

动植物源性产品

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