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D9163

DL-Dithiothreitol

Name: <SC>DL</SC>-Dithiothreitol
Brand: SIGMA

suitable for electrophoresis, ≥99%

Synonym(s):

threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

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About This Item

Linear Formula:

HSCH₂CH(OH)CH(OH)CH₂SH

CAS Number:

3483-12-3

Molecular Weight:

154.25

UNSPSC Code:

12161504

NACRES:

NB.22

PubChem Substance ID:

24894266

EC Number:

222-468-7

Beilstein/REAXYS Number:

1719757

MDL number:

MFCD00004877

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Quality Level

200

assay

≥99%

form

powder

reaction suitability

reagent type: reductant

technique(s)

electrophoresis: suitable

mp

40-44 °C, 41-44 °C (lit.)

solubility

H₂O: 50 mg/mL, clear, colorless to very faintly yellow

cation traces

heavy metals (as Pb): ≤10 ppm

absorption

≤0.04 at 280 at 0.02 M

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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Related Categories

Biochemicals

Chelating & Reducing Agents for Research

Chemical Synthesis

Chemistry & Biochemicals

Reducing Agents

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Analysis Note

Tested for use in reduced SDS-PAGE

pictograms

GHS05,GHS07

signalword

Danger

hcodes

H302,H315,H318

pcodes

P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Protein Turnover in Epithelial Cells and Mucus along the Gastrointestinal Tract Is Coordinated by the Spatial Location and Microbiota.

Liisa Arike et al.

Cell reports, 30(4), 1077-1087 (2020-01-30)

The gastrointestinal tract is covered by a single layer of epithelial cells that, together with the mucus layers, protect the underlying tissue from microbial invasion. The epithelium has one of the highest turnover rates in the body. Using stable isotope

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Neurology(R) neuroimmunology & neuroinflammation, 8(1) (2020-11-05)

To describe the clinical syndrome of 4 new patients with seizure-related 6 homolog like 2 antibodies (SEZ6L2-abs), study the antibody characteristics, and evaluate their effects on neuronal cultures. SEZ6L2-abs were initially identified in serum and CSF of a patient with

Salt-inducible kinase 2 regulates TFEB and is required for autophagic flux in adipocytes.

Florentina Negoita et al.

Biochemical and biophysical research communications, 508(3), 775-779 (2018-12-12)

Dysregulation of autophagy has been observed in obesity and type 2 diabetes. Salt-inducible kinase 2 (SIK2), a member of the AMPK-related kinase family, is downregulated in adipocytes from obese or insulin resistant individuals and was previously demonstrated to regulate autophagy

Unique requirements for mono- and polyubiquitination of the peroxisomal targeting signal co-receptor, Pex20.

Xueqian Liu et al.

The Journal of biological chemistry, 288(10), 7230-7240 (2013-01-25)

In Pichia pastoris, the peroxisomal targeting signal 2 (PTS2)-dependent peroxisomal matrix protein import pathway requires the receptor, Pex7, and its co-receptor Pex20. A conserved lysine (Lys(19)) near the N terminus of Pex20 is required for its polyubiquitination and proteasomal degradation

Antagonistic relationship between the unfolded protein response and myocardin-driven transcription in smooth muscle.

Baoyi Zhu et al.

Journal of cellular physiology, 235(10), 7370-7382 (2020-02-11)

Smooth muscle cells (SMCs) are characterized by a high degree of phenotypic plasticity. Contractile differentiation is governed by myocardin-related transcription factors (MRTFs), in particular myocardin (MYOCD), and when their drive is lost, the cells become proliferative and synthetic with an

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Global Trade Item Number

SKU	GTIN
D9163-25G	04061835521814
D9163-5G	04061833590393
D9163-1G	04061833590386

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