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Merck
CN

D9655

pBR322 Hae III Digest

for DNA electrophoresis

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About This Item

NACRES:
NA.25
UNSPSC Code:
12352202

Key Documents

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Product Name

pBR322 Hae III Digest, for DNA electrophoresis

form

liquid

usage

1000 uses (0.5 mg size)
200 uses (0.1 mg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

Quality Level

100

Related Categories

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

General description

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Preparation Note

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000451886
0000451886
0000424295
0000424295
SLCQ5563

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Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

选择用于凝胶电泳的DNA、RNA和PCR片段的标记物和分子量标准

选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

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