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Merck
CN

D9785

SAFC

DMEM/F-12

With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure

Synonym(s):

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

Key Documents

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Product Name

DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.

Preparation Note

Formulated to contain 14.8 grams of powder per liter of medium.
Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
0000278759
0000264744
0000278759
0000264744
SLCL5755

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Vincent J Munster et al.
Nature, 585(7824), 268-272 (2020-05-13)
An outbreak of coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus (named SARS-CoV-2) and has a case fatality rate of approximately 2%, started in Wuhan (China) in December 20191,2. Following an unprecedented global spread3, the World Health Organization
Yuuma Furutani et al.
Cell biochemistry and function, 29(7), 577-581 (2011-08-23)
Magnesium (Mg) deficiency induces the production of free radicals, increases cytosolic ionized calcium concentration, and modulates the function of skeletal muscle in rats. The present study examined the effects of Mg deficiency on the gene expression of molecules related to
Huntingtin promotes mTORC1 signaling in the pathogenesis of Huntington's disease.
Pryor WM, Biagioli M, Shahani N, et al.
Science Signaling, 7(349), ra103-ra103 (2014)
Rui Yan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 103, 1490-1497 (2018-06-06)
Neonatal brain hypoxia is a disease that affects the nervous system in children. Salidroside is a compound that has an anti-hypoxic effect, but the mechanism of salidroside in neonatal cerebral hypoxia is unclear. Hence, we investigated the regulatory effect and
Wei Ye et al.
Frontiers in microbiology, 11, 1105-1105 (2020-07-01)
Human enteroviruses are responsible for diverse diseases, from mild respiratory symptoms to fatal neurological complications. Currently, no registered antivirals have been approved for clinical therapy. Thus, a therapeutic agent for the enterovirus-related disease is urgently needed. Remdesivir (GS-5734) is a
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Articles

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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