DFF100
DEAE–Sepharose™
Fast Flow
Synonym(s):
Diethylaminoethyl–Sepharose™
form
suspension
technique(s)
affinity chromatography: suitable
matrix
6% cross-linked agarose
bead size
45-165 μm (wet)
pore size
~4,000,000 Da exclusion limit
pH
2—12
capacity
110-160 μeq/mL binding capacity(gel volume)
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General description
DFF100-500Ml′s update product number is GE17-0709-01
Application
DEAE-Sepharose has been used in:
- anion exchange chromatography
- to screen polyisoprenyl phosphate phosphatase activity
- to purify isoinhibitors
- for the purification of human immunodeficiency virus (HIV)
- glycoprotein envelope (gp140 env)
DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.
Biochem/physiol Actions
Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
100.4 - 109.4 °F
Flash Point(C)
38 - 43 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
危险化学品
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