DMN70
GenElute™ Direct mRNA Miniprep Kits
sufficient for 70 purifications
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General description
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.
Application
Features and Benefits
- mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
- Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
- Oligo(dT) polystyrene beads require fewer wash steps
Other Notes
Legal Information
Kit Components Only
- Collection tube 70 ea
- Elution solution 10 mL
- Filtration columns with tubes 70 ea
- Lysis solution 120 mL
- 5 M NaCl 8 mL
- Oligo(dT)-polystyrene beads 2 mL
- Proteinase K 25 mg
- Spin columns with tubes 70 ea
- 40% Glycerol solution 3 mL
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
Regulatory Information
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Articles
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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