DNTP100
Deoxynucleotide Set, 100 mM
Individual dNTPs for routine PCR; 0.25 mL each
Synonym(s):
DNTP Set for PCR
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About This Item
NACRES:
NA.52
UNSPSC Code:
41106305
Form:
liquid
Storage temp.:
−20°C
Product Name
Deoxynucleotide Set, 100 mM, Individual dNTPs for routine PCR; 0.25 mL each
form
liquid
concentration
100 mM
color
colorless
shipped in
dry ice
storage temp.
−20°C
Quality Level
Related Categories
Application
Deoxynucleotide Set, 100 mM has been used as a component of the reaction mixture for polymerase chain reaction (PCR).It has also been used as a component of the reaction mixture for amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to genotype single-nucleotide polymorphisms (SNPs).
Deoxynucleotide Set, 100 mM has been used:
- to biotinylate λ-DNA for magnetic tweezers measurements
- in RAPD PCR
- in FISH probe generation and labeling
- in PCR amplification of genomic DNA
- in multiplex PCR
- in first-strand cDNA synthesis
- in TUNEL labeling
- in RT-PCR
- as a component of the reaction mixture for polymerase chain reaction (PCR)
Biochem/physiol Actions
Deoxynucleotide triphosphates (dNTPs) are substrates for DNA polymerizing enzymes. They act as building blocks of nucleic acid molecules and are acidic in nature. dNTPs are key components of PCR master mixes and are highly essential to amplify DNA. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.
Features and Benefits
- Provided as 4 separate tubes of dNTPs.
- Allows optimization of individual dNTP concentrations.
General description
Deoxynucleotide triphosphates (dNTPs) are organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 0.25 mL each of 100mM aqueous solutions of four deoxyribonucleotides, deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.
Packaging
0.25 mL each of 100 mM dATP, dCTP, dGTP and dTTP
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Michael G Mohsen et al.
Nucleic acids research, 47(18), 9495-9501 (2019-09-11)
We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the
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