DUO92020
Duolink® In Situ PLA® Probe Anti-Human PLUS
Affinity purified Donkey anti-Human IgG (H+L)
Synonym(s):
in situ Proximity Ligation Assay, Protein Protein Interaction Kit
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About This Item
biological source
donkey (Polyclonal)
antibody form
affinity purified immunoglobulin (Secondary antibody)
product line
Duolink®
species reactivity
human
technique(s)
immunofluorescence: suitable, proximity ligation assay: suitable
suitability
suitable for brightfield, suitable for fluorescence
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting and more.
To perform a complete Duolink® PLA® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
PLA probe anti-Human reacts with whole molecule human IgG and the light chains of other human immunoglobulin?s. The PLA Probe Anti-Human Plus has minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, rat, and sheep serum proteins. A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Other Notes
- 5x PLA Probe Anti-Human PLUS - Donkey anti-human secondary antibody conjugated to oligonucleotide PLUS
- 1x Blocking Solution - Reagent for blocking of the sample
- 1x Antibody Diluent - For dilution of PLA probes and primary antibodies
See datasheet for more information.
Legal Information
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
Regulatory Information
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Articles
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Things to consider for preparation, setup and execution of the Duolink® assay protocol
包括建议和技巧、常见问题解答和基本故障排除的支持信息。
正确地准备、设置和执行 Duolink® PLA 实验方案需要考虑的因素。
Protocols
本实验方案描述如何使用Duolink® PLA邻位连接试剂，通过流式细胞术检测细胞群内的单个蛋白质、蛋白修饰和蛋白相互。
Related Content
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
利用邻位连接技术对蛋白质互作、翻译后修饰和低表达蛋白进行检测、定量和成像应用。
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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