Duolink®近接测定
Duolink® Proximity Ligation Assays (PLA)能够研究内源性蛋白质-蛋白质相互作用（PPI）和翻译后修饰，为单个事件提供荧光信号，可通过显微镜或流式细胞仪进行观察。为了研究蛋白质-蛋白质相互作用，Duolink® PLA 技术使用一对标记有寡核苷酸的抗体，当寡核苷酸存在于 40 纳米范围内时，在加入标记探针后进行滚圆放大，产生特定的荧光信号。
Duolink®聚乳酸有四种不同的颜色，适用于流式细胞仪和显微镜，可使每个细胞中的多种蛋白质-蛋白质相互作用可视化。利用 Duolink® PLA 技术的放大性能和优势，发掘您的研究机会：
- 观察蛋白质相互作用--稳定的和瞬时的
- 观察翻译后修饰
- 检测内源蛋白质--无需过度表达或基因操作
- 高特异性--使用两种抗体/探针可消除假阳性
- 无需特殊设备--可使用标准免疫荧光和流式细胞术方法
Products
Duolink®聚乳酸产品
- Duolink®聚乳酸起始试剂盒包含进行荧光实验和分析多达 30 个样品所需的全部试剂。
- Duolink®Probemaker试剂盒可将聚乳酸寡聚体（PLUS或MINUS）与任何抗体连接。
- Duolink®聚乳酸多色试剂盒可通过显微镜实现多种 PPI 视觉效果。
- Duolink®flowPLA 检测试剂盒可使用四种颜色（绿、橙、红和远红）进行流式细胞仪应用。
- Duolink®聚乳酸明视野检测包含用辣根过氧化物酶（HRP）标记的寡核苷酸，可与光学显微镜一起使用。
相关资源
- Application Note: An Introduction to Duolink® PLA
Duolink® PLA protein detection technology is based on in situ proximity ligation assay (PLA), and allows for the detection, visualization, and quantification of protein expression, protein-protein interactions, and post-translational modifications, in unmodified cells and tissue samples within a matter of hours.
- Creating PLA® Probes Protocol
Duolink®试剂盒使用原位PLA®技术（一种邻位连接检测技术）准确、客观地量化单个蛋白质，以及这些蛋白在未修饰细胞和组织中的互作和修饰。
- Article: Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
- Article: Duolink® PLA Flow Cytometry Kits
Duolink® PLA kit enhances flow cytometry for detecting protein interactions accurately.
- Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
- Duolink® PLA Fluorescence Protocol
This protocol describes the use of Duolink® PLA reagents for the immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
- Application Note: How to Optimize the Duolink® Proximity Ligation Assay
To successfully execute a Duolink® PLA experiment, there are some necessary considerations to properly prepare for, setup, and execute the assay protocol.
- Brochure: Amplified Detection Duolink® Proximity Ligation Assay (PLA)
Rely on Duolink® PLA to amplify protein signals by 1000x, allowing you to visually detect proteins and their interactions, all with a straightforward protocol.
- Application Note: Duolink® PLA Probemaker Guide
This guide describes the use of the Duolink® In Situ Probemaker for the conjugation of PLA oligonucleotides (PLUS or MINUS) to any antibody for use in Duolink® PLA experiments.
- Flyer: Duolink® Flow Cytometry
Traditional flow cytometry has been limited in the ability to detect protein interactions and low abundant proteins events — until now.
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