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Merck
CN

E0760

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

Synonym(s):

ECGS, Endothelial Mitogen

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
Biological source:
bovine pituitary glands
Form:
powder
Impurities:
≤20 EU/vial (LAL test)
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Product Name

Endothelial cell growth supplement from bovine pituitary, ECGS, BioReagent, suitable for cell culture

biological source

bovine pituitary glands

Quality Segment

product line

BioReagent

form

powder

potency

2-300 μg/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

General description

Endothelial Cell Growth Supplement (ECGS) is a BioReagent‑grade extract derived from bovine pituitary glands and contains growth‑promoting factors that support the growth and maintenance of vascular endothelial cells of mammalian origin. The supplement is prepared using a modified published method and supplied as a lyophilized powder from a sterile NaCl solution containing streptomycin sulfate. ECGS is endotoxin tested and designed for use in in vitro mammalian cell culture research.

Application

Endothelial Cell Growth Supplement (ECGS) from bovine pituitary is commonly used as a supplement to basal culture media for endothelial cell research applications, including:
  • RPMI‑1640 medium for mammalian endothelial cell culture
  • Medium 199 for endothelial cell growth and maintenance
  • DMEM:Ham’s F‑12 medium for endothelial cell culture
  • IMDM for human umbilical vein endothelial cell (HUVEC) culture
  • Supplementation of media in hybridoma fusion and growth studies

Biochem/physiol Actions

Endothelial Cell Growth Supplement (ECGS) provides a complex mixture of biologically derived growth‑promoting factors that support endothelial cell growth, attachment, and maintenance. ECGS has also been reported as a supportive media supplement in hybridoma cell fusion and growth workflows for monoclonal antibody research applications.


pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Repr. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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