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E1532

Monoclonal Anti-E2F6 antibody produced in mouse

Name: Monoclonal Anti-E2F6 antibody produced in mouse
Brand: SIGMA

~1.0 mg/mL, clone TFE61, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-E2F transcription factor 6, Anti-E2F-6

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

TFE61, monoclonal

Application:

ELISA (i), ICC, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TFE61, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using HeLa nuclear cell extract

isotype

IgG1

UniProt accession no.

O75461

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... E2F6(1876)

Description

General description

E2F6 transcription factor belongs to the third class of E2Fs. The E2F transcription factors are a family of genes that play critical roles in regulation of cellular proliferation and differentiation.

Immunogen

recombinant human E2F6 protein

Application

Monoclonal Anti-E2F6 antibody produced in mouse is suitable for immunocytochemistry and indirect ELISA. It is also suitable for western blotting at a working concentration of 2-4μg/mL using HeLa nuclear cell extract.

Biochem/physiol Actions

The third class of E2Fs lacks both the retinoblastoma protein binding and transcriptional activation domain, and is an active repressor of transcription. The activity of E2F6 is mediated through its recruitment of polycomb transcriptional repressor complexes in a histone methylation independent manner. E2F6 associates with E2F target genes that are activated at G1/S phase. Its repression activity during the S-phase distinguishes G1/S and G2/M transcription during cell cycle. It may be involved in various cancerous processes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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