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E1532

Monoclonal Anti-E2F6 antibody produced in mouse

~1.0 mg/mL, clone TFE61, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-E2F transcription factor 6, Anti-E2F-6

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
TFE61, monoclonal
Application:
ELISA (i), ICC, WB
Citations:
7
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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TFE61, monoclonal

form

buffered aqueous solution

mol wt

antigen ~35 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 2-4 μg/mL using HeLa nuclear cell extract

isotype

IgG1

UniProt accession no.

O75461

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... E2F6(1876)

General description

E2F6 transcription factor belongs to the third class of E2Fs. The E2F transcription factors are a family of genes that play critical roles in regulation of cellular proliferation and differentiation.

Immunogen

recombinant human E2F6 protein

Application

Monoclonal Anti-E2F6 antibody produced in mouse is suitable for immunocytochemistry and indirect ELISA. It is also suitable for western blotting at a working concentration of 2-4μg/mL using HeLa nuclear cell extract.

Biochem/physiol Actions

The third class of E2Fs lacks both the retinoblastoma protein binding and transcriptional activation domain, and is an active repressor of transcription. The activity of E2F6 is mediated through its recruitment of polycomb transcriptional repressor complexes in a histone methylation independent manner. E2F6 associates with E2F target genes that are activated at G1/S phase. Its repression activity during the S-phase distinguishes G1/S and G2/M transcription during cell cycle. It may be involved in various cancerous processes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

wgk

WGK 1

Storage Class

12 - Non Combustible Liquids

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
068K4789

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Related Content

Data Sheet - E1532

Instructions

Phillip J Iaquinta et al.
Current opinion in cell biology, 19(6), 649-657 (2007-11-23)
The E2F transcription factors are critical regulators of genes required for appropriate progression through the cell cycle, and in special circumstances they can also promote the expression of another class of genes that function in the apoptotic program. Since E2Fs
Gwen MacDonald et al.
Breast cancer research : BCR, 9(4), R49-R49 (2007-08-01)
Decreased transcription of the BRCA1 gene has previously been observed to occur in sporadic breast tumours, making elucidation of the mechanisms regulating the expression of this gene important for our understanding of the etiology of the disease. Transcriptional elements involved
Paloma H Giangrande et al.
Genes & development, 18(23), 2941-2951 (2004-12-03)
E2F transcription factors play a critical role in the control of cell cycle progression, regulating the expression of genes involved in DNA replication, DNA repair, mitosis, and cell fate. This involves both positive-acting and negative-acting E2F proteins, the latter group
View All Related Papers

Global Trade Item Number

SKUGTIN
E1532-200UL04061836265137