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E3406

Anti-EB1 antibody produced in rabbit

Name: Anti-EB1 antibody produced in rabbit
Brand: SIGMA

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-APC binding protein EB1, Anti-End-Binding-1, Anti-Microtuble-associated protein RP/EB family member 1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01633308

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35 kDa

species reactivity

rat, mouse, bovine, pig, canine, human

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line, indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells, indirect immunofluorescence: suitable, western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2

UniProt accession no.

Q15691

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPRE1(22919)
mouse ... Mapre1(13589)
rat ... Mapre1(114764)

Description

General description

Microtubule-associated protein RP/EB family member 1 is a protein encoded by the MAPRE1 gene in humans. EB1 (End-Binding-1) proteins belong to a distinct family of accessory proteins, termed “plus-end tracking proteins” or +TIPs, that bind to the plus-ends of microtubules. All EB1 family proteins have a highly conserved N-terminal region that contains a calponin homology (CH) domain and an EB1-like C-terminal motif, which is unique to EB1 family proteins.

Immunogen

synthetic peptide corresponding to amino acids 252-268 located at the C-terminus of human EB1, conjugated to KLH. This sequence is identical in several species (mouse, rat, dog, bovine, and porcine EB1), highly conserved (single amino acid substitution) in chicken EB1, and has limited homology to human EB2 (35% identity) and human EB3 (53% identity).

Application

Anti-EB1 antibody produced in rabbit has been used in

immunofluorescence
western blotting
immunocytochemistry
immunoprecipitation

Biochem/physiol Actions

End-Binding-1 (EB1) associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.

The C-terminal domain of End-binding proteins (EBs) is important for its autonomous microtubule tip tracking, regulation of microtubule dynamics, chromosome segregation and recruitment of numerous partners to microtubule ends. EB1 proteins bind to microtubule ends and act with the adenomatous polyposis coli (APC) tumor suppressor to regulate the microtubule filament system. It is a potential oncogene and plays an important role during early event in colon carcinogenesis, colorectal cancer (CRC). EB1 acts as a potential biomarker and therapeutic target in CRC.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)