E4643
Heparin-Binding EGF-Like Growth Factor human
≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
Synonym(s):
hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor
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About This Item
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Assay:
≥97% (SDS-PAGE and N-terminal analysis)
Form:
lyophilized powder
Mol wt:
9.5 kDa
Product Name
Heparin-Binding EGF-Like Growth Factor human, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in baculovirus infected Sf21 cells
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
≤3 ng/mL
quality
endotoxin tested
mol wt
9.5 kDa
packaging
pkg of 50 and 250 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
General description
Heparin‑Binding Epidermal Growth Factor‑Like Growth Factor (HB‑EGF) is a recombinant human member of the epidermal growth factor (EGF) family, expressed in baculovirus‑infected Sf21 insect cells and supplied as a lyophilized powder. HB‑EGF is produced with high purity (≥97%) and is suitable for use in mammalian cell culture and in vitro research applications.
Application
Recombinant human HB‑EGF is commonly used in cell‑based research applications, including: •Mammalian cell culture studies
•Growth and signaling studies in EGF‑responsive cell lines
•Cellular proliferation and migration assays in in vitro model systems
•Growth and signaling studies in EGF‑responsive cell lines
•Cellular proliferation and migration assays in in vitro model systems
Biochem/physiol Actions
Heparin‑Binding Epidermal Growth Factor‑Like Growth Factor (HB‑EGF) is a heparin‑binding member of the EGF growth factor family. Soluble HB‑EGF is a glycoprotein that exhibits mitogenic and chemotactic activity toward multiple cell types, including fibroblasts, keratinocytes, and smooth muscle cells, in vitro. HB‑EGF is produced by several cell types, including monocytes, macrophages, and vascular smooth muscle cells, and signals primarily through the EGF receptor (EGFR) with high affinity and ErbB family receptors with moderate affinity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
flash_point_f
No data available
flash_point_c
No data available
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