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Merck
CN

E8008

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

EDTA solution

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About This Item

Empirical Formula (Hill Notation):
C10H16N2O8
CAS Number:
60-00-4
Molecular Weight:
292.24
NACRES:
NA.75
PubChem Substance ID:
24894664
eCl@ss:
39030907
UNSPSC Code:
12352204
MDL number:
MFCD00003541
Beilstein/REAXYS Number:
1716295

Key Documents

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Product Name

Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

sterility

sterile-filtered

product line

BioReagent

form

solution

concentration

0.02% in DPBS (0.5 mM)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.1-7.7

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL7490
RNBL4916
RNBL0132
RNBK3718
RNBJ7436

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Ex vivo Lung bioengineering
Lung Stem Cells in the Epithelium and Vasculature, 151-151 (2015)
The genotoxicity of physiological concentrations of chromium (Cr (III) and Cr (VI)) and cobalt (Co (II)): an in vitro study
Figgitt M, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 688(1-2), 53-61 (2010)
Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
Wang Y, et al.
Stem Cell Research & Therapy, 8(1), 189-189 (2017)
Cerium fluoride nanoparticles protect cells against oxidative stress.
Shcherbakov AB, et al.
Materials Science and Engineering, C, 50, 151-159 (2015)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)
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