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E8145

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

≥97%

Synonym(s):

EGTA tetrasodium, Egtazic acid tetrasodium, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium

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About This Item

Empirical Formula (Hill Notation):
C14H20N2O10Na4
CAS Number:
13368-13-3
Molecular Weight:
468.28
NACRES:
NA.77
PubChem Substance ID:
24894667
UNSPSC Code:
12352202
MDL number:
MFCD00274420
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Quality Level

200

assay

≥97%

technique(s)

RNA purification: suitable

solubility

H2O: soluble 10%, clear, colorless

application(s)

life science and biopharma

SMILES string

[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O

InChI

1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4

InChI key

BAOGCDPNNOBWQZ-UHFFFAOYSA-J

Related Categories

Application

EGTA is suitable for use:
  • in media for chelating Ca2+ to facilitate efficient incorporation of Sr2+ into the oocytes
  • as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins
  • as a component of oocyte activation media
A chelating agent useful for the determination of calcium in the presence of magnesium.
A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca2+.

Biochem/physiol Actions

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).
Ethylene glycol-bis(β-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca2+ in the presence of Mg2+ at a ratio of 1:1.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000543510
0000545763
0000543510
0000545763
0000517330

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Sayaka Wakayama et al.
Methods in enzymology, 476, 151-169 (2010-08-10)
Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown
Shirley P and Saunders T L
Advanced Protocols For Animal Transgenesis, 271-277 (2011)
Schmid, R.W. et al.
Analytical Chemistry, 29, 264-264 (1957)
EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.
Zhang C, et al.
acta societatis botanicorum poloniae, 85(3) (2016)
EGTA treatment of human airways in vitro unmasks M1/MUC5AC mucin in submucosal glands
Roger P, et al.
The European Respiratory Journal, 18(1), 176-183 (2001)
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Related Content

Product Information Sheet - E8145

Product Information Sheet

Global Trade Item Number

SKUGTIN
E8145-10G04061833608647
E8145-50G04061833608654

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