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E8640

E-64d

Name: E-64d
Brand: SIGMA

powder, ≥98% (TLC)

Synonym(s):

(2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane ethyl ester, EST

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₃₀N₂O₅

CAS Number:

88321-09-9

Molecular Weight:

342.43

UNSPSC Code:

12352202

PubChem Substance ID:

24894681

NACRES:

NA.77

MDL number:

MFCD00132883

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Product Name

E-64d, protease inhibitor

biological source

synthetic (organic)

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

methanol: water (1:1): 5 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

CCOC(=O)[C@H]1O[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)NCCC(C)C

InChI

1S/C17H30N2O5/c1-6-23-17(22)14-13(24-14)16(21)19-12(9-11(4)5)15(20)18-8-7-10(2)3/h10-14H,6-9H2,1-5H3,(H,18,20)(H,19,21)/t12-,13-,14-/m0/s1

InChI key

SRVFFFJZQVENJC-IHRRRGAJSA-N

Gene Information

human ... CAPN1(823)

Application

E-64d has been used as a lysosomal inhibitor in human acute promyelocytic leukemia NB4 cells and Huh-7 cells. It has also been used as a protease inhibitor in free calcium physiological tyrode solution for perfusion into the isolated heart samples.

Biochem/physiol Actions

E-64d is an epoxysuccinyl peptide and an inhibitor of cysteine protease cathepsin B, calpains 1 and 2. E-64d by its cathepsin B protease inhibition functionality, may serve as a potential drug for treating traumatic brain injury (TBI) and Alzheimer′s disease (AD). It inhibits gametocyte surface antigen resulting in a decreased oocyst production in Plasmodium falciparum.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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From EST to novel spider silk gene identification for production of spidroin-based biomaterials.

Weidong Huang et al.

Scientific reports, 7(1), 13354-13354 (2017-10-19)

A cDNA library from a pool of all the seven silk glands from a tropical spider species was constructed. More than 1000 expressed sequence tag (EST) clones were created. Almost 65% of the EST clones were identified and around 50%

Ethambutol neutralizes lysosomes and causes lysosomal zinc accumulation

Yamada D, et al.

Biochemical and Biophysical Research Communications, 471(1), 109-116 (2016)

Evolutionary characteristics of intergenic transcribed regions indicate rare novel genes and widespread noisy transcription in the Poaceae.

John P Lloyd et al.

Scientific reports, 9(1), 12122-12122 (2019-08-23)

Extensive transcriptional activity occurring in intergenic regions of genomes has raised the question whether intergenic transcription represents the activity of novel genes or noisy expression. To address this, we evaluated cross-species and post-duplication sequence and expression conservation of intergenic transcribed

The cysteine protease inhibitor, E64d, reduces brain amyloid-beta and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B, but not BACE1, beta-secretase activity

Hook G, et al.

Journal of Alzheimer'S Disease, 26(2), 387-408 (2011)

Inhibition of Plasmodium falciparum oocyst production by membrane-permeant cysteine protease inhibitor E64d

Eksi S, et al.

Antimicrobial Agents and Chemotherapy, 51(3), 1064-1070 (2007)

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Global Trade Item Number

SKU	GTIN
E8640-1MG	04061833608791
E8640-250UG	04061833608807

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