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E8886

Sigma-Aldrich

Erythrosin extra bluish

certified by the Biological Stain Commission

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Synonym(s):
2′,4′,5′,7′-Tetraiodofluorescein disodium salt, Acid Red 51, Iodoeosin
Empirical Formula (Hill Notation):
C20H6I4Na2O5
CAS Number:
16423-68-0
Molecular Weight:
879.86
Colour Index Number:
45430
Beilstein:
1443945
EC Number:
240-474-8
MDL number:
MFCD00144257
PubChem Substance ID:
24894685
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥80%

pH

6.4 (30.3 °C)

mp

315 °C

solubility

water: 1 mg/mL, clear

density

0.625-0.731 g/cm3 at 30.1 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[O-]c1c(I)cc2c(Oc3c(I)c([O-])c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5.2Na/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20;;/h1-6,25-26H;;/q;2*+1/p-2

InChI key

RAGZEDHHTPQLAI-UHFFFAOYSA-L

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General description

Erythrosin extra bluish is soluble in ethanol and water. It is a brown powder, with a pH range of 2.5 to 4. It is used in color filters, light emitting diodes, cosmetics and hair dyes. It has been used in many biological applications such as, detecting gene expression, treating age related macular degeneration, diabetes and obesity.

Suitability

Certified for use in the Jackson stain for plant anatomy; counterstain for alum hematoxylin; Kreyberg′s method for keratin and mucus.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H302 - H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

359.6 °F - closed cup

Flash Point(C)

182 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R W Sabnis.
Handbook of Acid-Base Indicators (2007)
R W Sabnis.
Handbook of Biological Dyes and Stains: Synthesis and Industrial Applications, 544-544 (2010)
Ying-Ju Huang et al.
ChemMedChem, 7(4), 571-577 (2012-02-23)
SecA is a central component of the general secretion system that is essential for bacterial growth and thus an ideal target for antimicrobial agents. A series of fluorescein analogues were first screened against the ATPase activity using the truncated unregulated
Anna Carolina Borges Pereira Costa et al.
Mycoses, 55(1), 56-63 (2011-06-15)
The aim of this study was to evaluate the effects of photodynamic therapy (PDT) using rose bengal or erythrosine with light emitting diode (LED) on Candida albicans planktonic cultures and biofilms. Seven C. albicans clinical strains and one standard strain
Juliana P M L Rolim et al.
Journal of photochemistry and photobiology. B, Biology, 106, 40-46 (2011-11-11)
Several photosensitizers have been used against oral bacteria without standardization. Singlet oxygen ((1)O(2)) is an aggressive chemical species that can kill cells through apoptosis or necrosis. to compare the antimicrobial activity of photodynamic therapy (PDT) with different photosensitizers at the
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