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Merck
CN

E9653

Anti-Endothelial Cells antibody, Mouse monoclonal

clone P1H12, purified from hybridoma cell culture

Synonym(s):

Anti-CD146

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03792419

Key Documents

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Product Name

Anti-Endothelial Cells antibody, Mouse monoclonal, clone P1H12, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P1H12, monoclonal

form

buffered aqueous solution

mol wt

antigen 130 kDa

species reactivity

human

technique(s)

flow cytometry: suitable using 2% paraformaldehyde 9:1 methanol
immunocytochemistry: suitable using 4% paraformaldehyde 0.1-0.5% triton X-100, smears or live cells
immunohistochemistry: suitable using using acetone-fixed sections of human tongue
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Related Categories

Application

Anti-Endothelial Cells antibody, Mouse monoclonal has been used in:
  • immunocytochemistry
  • immunohistochemistry
  • flow cytometry
  • immunoprecipitation
  • enzyme-linked immunosorbent assay (ELISA)
  • western blotting

Biochem/physiol Actions

CD146 (cluster of differentiation 146) expression can stimulate tumor development in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells during implantation and placentation, thus limiting trophoblastic invasion in the myometrium.
Recognizes cultured microvascular and large-vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation.
Soluble CD146 (cluster of differentiation 146) is associated with the activation of angiogenesis. Circulating endothelial cells (CECs) may help in predicting clinical outcome in cancer patients. Tumor endothelial markers are expressed in a wide range of tumor types and in normal vessels associated with wound healing and corpus luteum formation. It may have significant implications for the development of anti-angiogenic therapies as well.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Endothelial cells (CD146) (mouse IgG1 isotype) is derived from the P1H12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with human umbilical cord cells (HUVEC). CD146 (cluster of differentiation 146) is also known as the melanoma cell adhesion molecule (MCAM), cell surface glycoprotein MUC18, A32, Mel-CAM and S-Endo-1. It is encoded by the MCAM gene. It belongs to the immunoglobulin supergene family with five immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. It is located on human chromosome11q23.

Immunogen

human umbilical cord cells (HUVEC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
018M4891
22170951
032K4894

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Xianyi Yu et al.
Pediatric research, 55(4), 688-694 (2004-02-07)
Nitric oxide (NO) serves many vasoprotective roles, but the massive release of NO causes arterial wall degeneration. We investigated whether enhanced nitric oxide synthase (iNOS) expression in peripheral blood leukocytes and circulating endothelial cells mirrors the progression of coronary arterial
M Ilie et al.
British journal of cancer, 110(5), 1236-1243 (2014-01-30)
Previous studies indicate that endothelial injury, as demonstrated by the presence of circulating endothelial cells (CECs), may predict clinical outcome in cancer patients. In addition, soluble CD146 (sCD146) may reflect activation of angiogenesis. However, no study has investigated their combined
Targeting CD146 with a 64Cu-labeled antibody enables in vivo immunoPET imaging of high-grade gliomas
Yang Y, et al.
Proceedings of the National Academy of Sciences of the USA, 112(47), E6525-E6534 (2015)
Expression of CD markers in JAK2V617F positive myeloproliferative neoplasms: Prognostic significance
Shahrabi S, et al.
Oncology reviews, 12(2) (2018)
The role of CD146 (Mel-CAM) in biology and pathology
Shih IM
The Journal of Pathology, 189(1), 4-11 (1999)
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Related Content

Data Sheet - E9653

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