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Merck
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ESPCAS9PRO

eSpCas9 Protein

from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS

Synonym(s):

Enhanced specificity Cas9, eCas9, eSpCas9, eSpCas9 1.1, eSpyCas9

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About This Item

NACRES:
NA.51
UNSPSC Code:
12352202

Key Documents

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Product Name

eSpCas9 Protein, from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

lyophilized powder

packaging

pkg of 1 kit (3 components)

application(s)

CRISPR

shipped in

ambient

storage temp.

−20°C

Quality Level

300

Related Categories

Application

Functional Genomics/Target Validation/Genome Editing

Biochem/physiol Actions

Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.

Features and Benefits

  • Enhanced specificity compared to wild type Cas9
  • Highly active
  • Ready-to-inject/transfect

General description

Recombinant eSpCas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.

Legal Information

CRISPR Use License Agreement
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Each kit consists of:
  • one vial of eSpCas9 recombinant protein
  • one vial containing 1 mL of 1× dilution buffer
  • one vial containing 1 mL of nuclease-free water with glycerol
Use our CRISPR Selection Tool to order gRNA

Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins

Packaging

pkg of 50 μg
pkg of 250 μg

Preparation Note

Lyophilized S. pyogenes eSpCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration for storage. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000341435
0000154434
059M4061V
0000154434
0000341435

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Evan R Stark-Dykema et al.
Scientific reports, 12(1), 8554-8554 (2022-05-21)
Mammalian sex chromosomes are enriched for large, nearly-identical, palindromic sequences harboring genes expressed predominately in testicular germ cells. Discerning if individual palindrome-associated gene families are essential for male reproduction is difficult due to challenges in disrupting all copies of a
CRISPR off-target analysis in genetically engineered rats and mice
Keith R. Anderson, et al
Nature Methods (2018)
Vasin Dumrongprechachan et al.
eLife, 11 (2022-10-15)
Mammalian axonal development begins in embryonic stages and continues postnatally. After birth, axonal proteomic landscape changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Comprehensive profiling of axonal proteomes across neurodevelopment is limited, with most studies lacking cell-type and
CRISPR/Cas9 Endonuclease-Mediated Mouse Genome Editing of One-Cell and/or Two-Cell Embryos by Electroporation, and the Use of Rad51 to Enhance Knock-In Allele Homozygosity via Interhomolog Repair Mechanism.
Garza, et al.
Methods in Molecular Biology, 2631, 253-266 (2023)
Yuki Matsushita et al.
Nature communications, 14(1), 2383-2383 (2023-05-15)
The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. It is well-described that leptin receptor (LepR)+ perivascular stromal cells provide a major source of bone-forming osteoblasts in
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