ESPCAS9PRO
eSpCas9 Protein
from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS
recombinant
expressed in E. coli
Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
packaging
pkg of 1 kit (3 components)
application(s)
CRISPR
shipped in
ambient
storage temp.
−20°C
Related Categories
General description
Application
Features and Benefits
- Enhanced specificity compared to wild type Cas9
- Highly active
- Ready-to-inject/transfect
Packaging
pkg of 250 μg
Components
- one vial of eSpCas9 recombinant protein
- one vial containing 1 mL of 1× dilution buffer
- one vial containing 1 mL of nuclease-free water with glycerol
Principle
Reconstitution
Other Notes
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Legal Information
Kit Components Only
- Enhanced specificity Cas9-NLS from Streptococcus pyogenes, expressed in Escherichia coli
- Dilution buffer for Cas9 proteins
- Reconstitution solution for Cas9 proteins
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
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Articles
After you have performed a CRISPR experiment it is important to determine which gRNAs performed successfully editing. There are many ways to validate CRISPR gene editing experiments. A quick and easy way to check for cutting is by using the Sigma-Aldrich® T7E1 mismatch detection kit.
Protocols
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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