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Merck
CN

F0804

Sigma-Aldrich

Fetal Bovine Serum, Research Grade

non-USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Bovine Serum, Fetal Bovine Sera, Fetal Calf Sera, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71

Key Documents

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Technical Service
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biological source

bovine fetus

Quality Level

400
500

sterility

sterile-filtered

product line

FBS Classic

composition

hemoglobin (tested)

origin

non-USA origin

availability

available only in EU

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested
 mycoplasma, tested
 virus (BVD, IBR, PI3), tested negative

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal bovine serum (FBS), a popular supplement, is mainly sourced from unborn calves. It contains nutrients, vitamins, hormones, transport proteins, and attachment, spreading, and growth factors.

Application

Fetal Bovine Serum, Research Grade has been used as a supplement:
  • of endothelial cell medium (ECMed) for culturing human umbilical vein endothelial cells (HUVEC)
  • in Dulbecco′s modified Eagle′s medium (DMEM) to resuspend human adipose tissue-derived stromal cells (ASC)
  • in the tissue culture medium 199 (TCM199) for in vitro oocyte maturation
  • in minimum essential medium (MEM) Earle′s medium for culturing Madin-Darby canine kidney (MDCK) II cells

Biochem/physiol Actions

Fetal bovine serum (FBS) is mainly used in organ, cell, and tissue culture. FBS is the first choice to be used as a supplement in all eukaryotic cell culture media. It supports the proliferation and viability of most cell types.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCC4867
BCCC4773
BCCC2250
BCCB7649
BCCB7351

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Jan van der Valk
Science (New York, N.Y.), 375(6577), 143-144 (2022-01-14)
Ethical and possible reproducibility issues arise when using fetal bovine serum in cell culture media.
Kayleigh M van Megen et al.
Frontiers in immunology, 10, 694-694 (2019-04-20)
Mesenchymal stromal cells (MSCs) are inherently immunomodulatory through production of inhibiting soluble factors and expression of immunosuppressive cell surface markers. We tested whether activated MSCs qualify for the induction of antigen-specific immune regulation. Bone marrow derived human MSCs were activated
Simona Ranallo et al.
Nature communications, 8, 15150-15150 (2017-05-10)
A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we
J van der Valk et al.
Toxicology in vitro : an international journal published in association with BIBRA, 18(1), 1-12 (2003-11-25)
Fetal bovine serum (FBS) is a common supplement to in vitro culture media. A workshop was organized to discuss whether or not fetuses might suffer when blood is withdrawn, and to discuss serum replacement methods. When bovine fetuses are exposed
Sara Kaliman et al.
Frontiers in physiology, 7, 551-551 (2016-12-10)
It is well accepted that cells in the tissue can be regarded as tiles tessellating space. A number of approaches were developed to find an appropriate mathematical description of such cell tiling. A particularly useful approach is the so called
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Articles

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

胎牛血清 (FBS) – 基础知识

回顾使用胎牛（或小牛）血清进行细胞培养的基础知识，以及采购和法规如何影响细胞测定所用血清的价格。

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