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About This Item
Linear Formula:
C15H14N3O2 · 0.5 ZnCl4
CAS Number:
Molecular Weight:
371.89
EC Number:
238-775-4
UNSPSC Code:
12171500
PubChem Substance ID:
Colour Index Number:
37165
MDL number:
InChI key
KIWIFIXMWIMRBZ-UHFFFAOYSA-L
InChI
1S/2C15H13N3O2.4ClH.Zn/c2*1-10-8-13(18-16)14(20-2)9-12(10)17-15(19)11-6-4-3-5-7-11;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
SMILES string
[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(NC(=O)c2ccccc2)c(C)cc1[N+]#N.COc3cc(NC(=O)c4ccccc4)c(C)cc3[N+]#N
composition
Dye content, ≥97%
storage temp.
2-8°C
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General description
Fast Violet B Salt is a diazonium salt. It is mainly required for cytochemical staining methods, for instance, alkaline phosphatase in leukocytes.
Application
Fast Violet B Salt has been used:
- for alkaline phosphatase staining in cells
- for hexosaminidase staining in cells
- to study lysosomal membrane stability
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Integrated coastal monitoring of a gas processing plant using native and caged mussels.
Brooks S
The Science of the Total Environment, 426, 375-375 (2012)
Turgeon ML
Clinical Hematology: Theory and Procedures (2005)
Beta-glucuronidase and hexosaminidase are marker enzymes for different compartments of the endo-lysosomal system in mussel digestive cells.
Izagirre U
Cell and Tissue Research, 335, 441-441 (2009)
W Cockburn et al.
Analytical biochemistry, 189(1), 95-98 (1990-08-15)
A sensitive, quantitative assay for phosphenolpyruvate carboxylase which utilizes microtiter plates is described. The assay depends upon the production of a colored compound in the reaction between oxaloacetate, the product of the phosphoenolpyruvate reaction, and the dye Fast Violet B.
Jean Rivoal et al.
Analytical biochemistry, 300(1), 94-99 (2001-12-18)
We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance
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