F1757
Fumarase from porcine heart
ammonium sulfate suspension, ≥300 units/mg protein (biuret)
Synonym(s):
L-Malate hydrolyase, Fumarate hydratase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-887-7
MDL number:
Product Name
Fumarase from porcine heart, ammonium sulfate suspension, ≥300 units/mg protein (biuret)
form
ammonium sulfate suspension
specific activity
≥300 units/mg protein (biuret)
foreign activity
malic dehydrogenase ≤0.1%
storage temp.
2-8°C
Quality Level
Application
Fumarase from porcine heart has been used:
- as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
- for immobilized enzyme based stability assay
- in fumarate hydratase assays in human lung carcinoma cells (A549)
Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.
Biochem/physiol Actions
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.
General description
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.
Other Notes
One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol
Preparation Note
Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
动植物源性产品
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E V Chernigovskaia et al.
Problemy endokrinologii, 34(1), 60-64 (1988-01-01)
The reaction of neurosecretory cells of some hypothalamic centers of rats of different age to experimental change of the blood level of corticoids to elucidate their role in the regulation of function of the hypophyseoadrenocortical system was studied. Immunohistochemical methods
Fumarase: a mitochondrial metabolic enzyme and a cytosolic/nuclear component of the DNA damage response
Yogev O, et al.
PLoS Biology, 8(3), e1000328-e1000328 (2010)
Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases.
Miettinen M, et al.
American Journal of Surgical Pathology, 40(12), 1661-1669 (2016)
Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid
Giorno L, et al.
Biotechnology and Bioengineering, 72(1), 77-84 (2001)
Fumarase: a paradigm of dual targeting and dual localized functions
Yogev O, et al.
FEBS Journal, 278(22), 4230-4242 (2011)
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
Protocols
Enzymatic Assay of Fumarase
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