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F1804

ANTI-FLAG^® M2 antibody, Mouse monoclonal

Name: ANTI-FLAG<SUP>®</SUP> M2 antibody, Mouse monoclonal
Brand: SIGMA

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):

flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG^® M2

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

M2, monoclonal

Application:

—

Citations:

9676

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1.0-1.2 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

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Related Categories

FLAG Purification

Protein Purification Chromatography

Protein Sample Prep

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG^® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

10 - Combustible liquids

Regulatory Information

低风险生物材料

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Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis.

Monika Srivastava et al.

Nature communications, 6, 6253-6253 (2015-02-24)

Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances

HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway

Yalan Liu

Virology, 154-154 (2016)

SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress.

Yan Han et al.

The Journal of biological chemistry, 285(17), 12906-12915 (2010-02-26)

Small ubiquitin-like modifier (SUMO) 2/3 is known to conjugate to substrates in response to a variety of cellular stresses. However, whether and how SUMO2/3-specific proteases are involved in de-conjugation under cell stress is unclear. Here, we show that low doses

MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBP?

Barbara Lipert

PLoS ONE (2017)

TRIM50 acts as a novel Src suppressor and inhibits ovarian cancer progression.

Yumin Qiu et al.

Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)

Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as

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Global Trade Item Number

SKU	GTIN
F1804-1MG	04061838261748
F1804-200UG	04061833612491
F1804-50UG	04061838261755
F1804-5MG	04061838261762

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