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F2168

Sigma-Aldrich

Anti-α-Tubulin−FITC antibody, Mouse monoclonal

clone DM1A, purified from hybridoma cell culture

Synonym(s):
Monoclonal Anti-α-Tubulin
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DM1A, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

bovine, rat, yeast, human, mouse, chicken, fungi, amphibian

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:50 using cultured BHK cells

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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General description

α/β-Tubulin, the major building block of microtubules, belongs to the tubulin superfamily of proteins. They are ubiquitously found in all eukaryotic cells. It exists in multiple isotype in many organisms.† It is a heterodimer with intracellular, hollow, cylindrical, filamentous structures. It is located on human chromosome 2q35. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β--Tubulin is a heterodimer, which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog. Tubulin is a highly conserved protein with two terminal domains: large N and C terminal.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC.

Specificity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.
The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

Immunogen

microtubules from chicken embryo brain.
Microtubules from chicken embryo brain.

Application

Monoclonal Anti-α-Tubulin antibody is suitable for immunostaining of:
  • respiratory epithelium tissue in a study to determine the tubulin expression in the mice cilia
  • breast cancer tissue sections to study the effect of LMO4 on the centrosome amplification and mitotic spindle abnormalities
  • spindle and chromosomes of oocytes
Mouse monoclonal antibody has been used in the:
  • detection of tubulin by immunofluorescence and confocal microscopy in lung carcinoma cell.
  • immunofluorescent staining of microtubules in human embryos and mitotic spindles from spleen lymphoblast.

Biochem/physiol Actions

The N-terminal domain of α−tubulin binds GTP, which is non-exchangeable mechanism, whereas highly acidic C-terminal domain is capable to bind microtubule-associated proteins (MAPs). Both domains are associated with the microtubular self-assembly. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. Mutations in tubulin α-4A gene leads to a neurodegenerative disease called amyotrophic lateral sclerosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

Storage and Stability

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing , or storage in "frost-free" freezers. is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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