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F2293

Sigma-Aldrich

Follicle Stimulating Hormone from porcine pituitary

Synonym(s):

FSH, Follitropin, pFSH

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About This Item

CAS Number:
9002-68-0
MDL number:
MFCD00131099
UNSPSC Code:
12352209
eCl@ss:
42020504
NACRES:
NA.32
Biological source:
Porcine
Form:
powder

Key Documents

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biological source

Porcine

form

powder

packaging

pkg of 50 unit per vial

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL

UniProt accession no.

P01228
P23945

storage temp.

2-8°C

InChI

1S/C79H125N19O23S/c1-39(2)34-53(90-67(110)51(27-29-57(102)103)87-66(109)49(20-14-15-31-80)86-70(113)54(35-45-18-12-11-13-19-45)92-76(119)63(43(9)99)96-65(108)48(81)38-122)72(115)93-60(40(3)4)75(118)91-55(36-46-23-25-47(101)26-24-46)71(114)88-52(28-30-58(104)105)69(112)97-64(44(10)100)77(120)94-61(41(5)6)74(117)89-50(21-16-32-84-79(82)83)68(111)95-62(42(7)8)78(121)98-33-17-22-56(98)73(116)85-37-59(106)107/h11-13,18-19,23-26,39-44,48-56,60-64,99-101,122H,14-17,20-22,27-38,80-81H2,1-10H3,(H,85,116)(H,86,113)(H,87,109)(H,88,114)(H,89,117)(H,90,110)(H,91,118)(H,92,119)(H,93,115)(H,94,120)(H,95,111)(H,96,108)(H,97,112)(H,102,103)(H,104,105)(H,106,107)(H4,82,83,84)/t43-,44-,48+,49+,50+,51+,52+,53+,54+,55+,56+,60+,61+,62+,63+,64+/m1/s1

InChI key

RPJRYFRWJUYJGY-AYWRSWFDSA-N

Gene Information

human ... FSHR(2492)
pig ... FSHB(396895)

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General description

FSH (follicle stimulating hormone) protein is composed of two subunits- α and β, where α subunit has resemblance to LH, hCG and TSH, which are glycoprotein hormones. α subunit has one glycosylation site and β subunit has two.

Application

Follicle stimulating hormone from porcine pituitary has been used for the in vitro maturation, fertilization and co-culture of cumulus oocyte complex and the co-culture of primary germ and Sertoli cells obtained from adult rat testes.

Biochem/physiol Actions

FSH in involved in the growth of ovarian follicles and steroidogenesis of ovaries. FSHR (follicle stimulating hormone receptor) interacts with FSH in the ovaries, and determines the responsiveness to this hormone. Depressed levels of FSHR result in poor responsiveness and influence folliculogenesis. In Caucasian population, certain polymorphisms in this gene are more commonly found in patients with polycystic ovary syndrome (PCOS).
The α-chain is not active; biological specificity is attributed to the β-chain. Induces maturation of Graafian follicles of the ovary; promotes development of germinal cells in males; activates cytosolic tyrosine kinase.

Other Notes

One unit is the activity of 1 mg of Armour FSH Standard G-94 by the method of Steelman, S.L., and Pohley, F.M., Endocrinology, 53, 604 (1953).
Two-chain glycoprotein hormone

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBR3807V
SLBP7214V
SLBL7543V
SLBK2330V
SLBJ5226V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. For Product F2293, Follicle Stimulating Hormone, do you have information on reconstitution, solution storage and potency?

    Product F2293-1VL is packaged as 50 units/vial. Each vial contains roughly 35-36 milligrams solid. Our solubility testing is done at 50 mg/mL, which yields a slightly hazy solution. It may be more convenient to use units/mL for your experiment as it will be more precise. If you decide to try to weigh out the material, please realize that small quantities can be difficult to weigh accurately.For Product F2293, FSH from human pituitary, we recommend solution storage for up to one month at 2-8°C. Unfortunately, we do not have any Sigma data regarding the stability of the porcine hormone.In the absence of information, we would recommend that solutions of Product F2293 not be frozen. If you do decide to store the product at -20°C we would suggest storing it in single use aliquots to avoid numerous freeze/thaw cycles.For the human FSH product we list an approximate activity of 7000 International Units/milligram. This should provide you with a starting point for a conversion calculation. For the porcine product we do not provide a conversion for units to mass. We provide 50 Armour units per vial. The unit definitions are also slightly different. International units are based on an international standard (IRP-68/140) while Armour units are based on Armour FSH standard G-94. According to the literature, 1 Armour unit can contain 9.4-14.2 international units.

  6. Do you have information on the solution stability of Follicle Stimulating Hormone (FSH) from pituitary?

    For information on the solution stability of FSH (and much more), refer to Chem. Soc. Rev., 2(4), 355-395 (1973).From page 369:  "The stabilities of the most highly purified preparations of certain hormonal glycoproteins have presented some problems, e.g. follicle-stimulating hormone preparations are often found to be stable in frozen solution but lose activity dramatically on concentration and freeze drying." Based on that information, we would recommend that if you need to freeze solutions of FSH, you should flash-freeze the solution (using either liquid nitrogen or a dry ice-alcohol bath), and store the solution in the ultra-cold (-70 or -80°C) freezer. Since FSH does have some solution stability concerns, simply placing the solution in a -20°C freezer without first flash-freezing may reduce the activity.

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    Ask a Scientist here.

M Evecen et al.
Theriogenology, 75(9), 1647-1651 (2011-03-15)
There have not been successful and repeatable methods for in vitro embryo production in the dog. Up to date, only one blactocyst has been achieved on in vitro culture. Since reproductive physiology of the dog is different from that of
K W Park et al.
Biology of reproduction, 65(6), 1681-1685 (2001-11-22)
The in vitro developmental potential of porcine nuclear transfer (NT) embryos was evaluated. Oocytes were matured for 42-44 h, and metaphase II-oocytes were enucleated. Fetal fibroblasts infected with the enhanced green fluorescent protein (EGFP) gene were serum-starved for 3-5 days.
Wanakan Singhasena et al.
Reproductive biology and endocrinology : RB&E, 12, 86-86 (2014-09-03)
Polymorphisms at codons 307 and 680 are the most commonly encountered allelic variants of the follicle-stimulating hormone receptor (FSHR) gene. Studies in Caucasians suggest that certain FSHR variants are more common in women with polycystic ovary syndrome (PCOS) than normal
Michele Morelli et al.
Gynecologic oncology, 129(3), 448-451 (2013-04-06)
The objective of this study is to compare ovarian function and surgical outcomes between patients affected by benign uterine pathologies submitted to total laparoscopic hysterectomy (TLH) plus salpingectomy and women in which standard TLH with adnexal preservation was performed. We
Kathryn S Timberlake et al.
Fertility and sterility, 100(6), 1735-1739 (2013-09-24)
To assess whether blood type was associated with diminished ovarian reserve (DOR) (day-3 follicle-stimulating hormone level >10 IU/L), controlling for history of tobacco smoking, body mass index (BMI), history of endometriosis, ovarian surgery, previous pregnancy, and maternal age. Cross-sectional study.
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