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F2442

Fetal Bovine Serum

Name: Fetal Bovine Serum
Brand: SIGMA

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

UNSPSC Code:

12352207

eCl@ss:

32160409

NACRES:

NA.71

MDL number:

MFCD00132239

Biological source:

bovine fetus

Origin:

USA origin

Sterility:

sterile-filtered

Shipped in:

dry ice

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biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Related Categories

Bovine Serum Albumin

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Fetal Bovine Serum (FBS)

Molecular Biology & Functional Genomics

General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media. FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system. FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Application

Fetal Bovine Serum has also been used as a component of:

perivascular-resident macrophage-like melanocytes (PVM/M) growth medium
synthetic oviduct fluid (SOF)
Eagle′s minimum essential medium (EMEM)
neurobasal medium
Hyclone McCoys 5 A medium
Hanks medium

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Analysis Note

Endotoxin and hemoglobin tested

Tested for the presence of bacteria, virus, and mycoplasma
Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

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Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.

Wie SM et al.

The Journal of Biological Chemistry, 289, 10900-10900 (2014)

Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium

Mosa FM, et al.

Ife Journal of Science, 4B, 3088-3093 (2015)

MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.

Grigoryev SA et al.

The Journal of Biological Chemistry, 274, 5626-5626 (1999)

Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.

van der Valk J et al.

Toxicology in vitro, 24, 1053-1053 (2010)

Species-specific vulnerability of RanBP2 shaped the evolution of SIV as it transmitted in African apes.

Nicholas R Meyerson et al.

PLoS pathogens, 14(3), e1006906-e1006906 (2018-03-09)

HIV-1 arose as the result of spillover of simian immunodeficiency viruses (SIVs) from great apes in Africa, namely from chimpanzees and gorillas. Chimpanzees and gorillas were, themselves, infected with SIV after virus spillover from African monkeys. During spillover events, SIV

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Global Trade Item Number

SKU	GTIN
F2442-100ML	04061838338709
F2442-24X500ML	04061838255372
F2442-1L	04061837034589
F2442-50ML	04061838255396
F2442-500ML	04061838255389
F2442-6X500ML	04061838255402

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