F2555
rabbit
unconjugated
ascites fluid
primary antibodies
SIG1-25, monoclonal
immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein
IgG
DYKDDDDK
dry ice
−20°C
10 - Combustible liquids
WGK 3
Not applicable
Not applicable
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The main application for Product F2555 rabbit monoclonal is for immunoblotting after immunoprecipitation with M2, which is a mouse antibody. This enables using a secondary antibody (anti-rabbit) that will not bind the M2 mouse heavy (50 kD) and light chains (25 kD).
F2555 will recognize N-terminal and C-terminal FLAG fusion proteins.
Two of the FLAG-tagged proteins offered by Sigma-Aldrich are the Amino-terminal FLAG-BAP fusion protein (Product No. P7582) and the Carboxy-terminal FLAG-BAP fusion protein (Product No. P7457). Both of these proteins should work with the F2555 rabbit ANTI-FLAG.
F2555 is a monoclonal antibody, raised in rabbits. [Note: Nearly all of the monoclonal antibodies sold by Sigma-Aldrich are raised in mice.]
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