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Merck
CN

F2756

Fluorescein di(β-D-galactopyranoside)

Synonym(s):

FDG

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About This Item

Empirical Formula (Hill Notation):
C32H32O15
CAS Number:
17817-20-8
Molecular Weight:
656.59
NACRES:
NC.07
PubChem Substance ID:
24894829
UNSPSC Code:
12352201
MDL number:
MFCD00077344

Key Documents

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InChI

1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1

SMILES string

OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O

InChI key

ZTOBILYWTYHOJB-WBCGDKOGSA-N

assay

≥98% (HPLC)

form

powder

solubility

methanol: 20 mg/mL, clear, yellow

storage temp.

−20°C

Quality Level

100

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Application

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Biochem/physiol Actions

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

General description

Fluorogenic substrate for β-galactosidase

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
17796LH
117H5116
087K5320
102K4612
095K5304

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N K Grzimek et al.
Journal of virology, 73(6), 5043-5055 (1999-05-11)
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Dominik Berzaczy et al.
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The response evaluation criteria in lymphoma (RECIL) classification for lymphoma treatment response assessment was introduced in 2017, but it has not yet been compared to the established Lugano classification. Also, the value of the provisional "minor response" (MiR) category of
A fluorimetric method using fluorescein di-?-D-galactopyranoside for quantifying the senescence-associated ?-galactosidase activity in human foreskin fibroblast Hs68 cells.
Yang NC and Hu ML
Analytical Biochemistry, 325, 337-343 (2004)
Alison H Clifford et al.
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