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Merck
CN

F3008

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source

sheep

Quality Level

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:32

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG) is available in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is a glycoprotein, with 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds.

Application

Anti-Mouse IgG (whole molecule)−FITC antibody produced in sheep has been used in:
  • flow cytometry
  • immunocytochemistry
  • immunofluorescent staining

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
常规特殊物品
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M A Zimmermann et al.
Haemophilia : the official journal of the World Federation of Hemophilia, 20(3), e215-e221 (2014-03-08)
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Journal of cell science, 117(Pt 22), 5233-5243 (2004-09-30)
The outer epidermal layer of the skin is an epithelium with remarkable protective barrier functions, which is subject to pronounced physical stress in its day-to-day function. A major candidate component for absorbing this stress is the K5/K14 keratin intermediate filament
Liang Liu et al.
Antioxidants (Basel, Switzerland), 11(8) (2022-08-27)
BTB and CNC homology 1 (BACH1) represses the expression of genes involved in the metabolism of iron, heme and reactive oxygen species and promotes metastasis of various cancers including pancreatic ductal adenocarcinoma (PDAC). However, it is not clear how BACH1
Alan Renton et al.
Carcinogenesis, 24(7), 1177-1182 (2003-06-17)
The p53 tumour suppressor gene is a transcription factor that can induce cell cycle arrest and apoptosis. In response to various stress-inducing signals, p53 level increases and this is accompanied with increased activities of p53. Interestingly, the methylxanthine caffeine can

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