F3022
Anti-β-Actin–FITC antibody, Mouse monoclonal
clone AC-15, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-β-Actin
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
AC-15, monoclonal
Application:
direct immunofluorescence
Species reactivity:
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
Citations:
23
Technique(s):
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
Uniprot accession no.:
Product Name
Anti-β-Actin–FITC antibody, Mouse monoclonal, clone AC-15, purified from hybridoma cell culture
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
AC-15, monoclonal
form
buffered aqueous solution
mol wt
antigen 42 kDa
species reactivity
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
should not react with
Dictyostelium discoideum
technique(s)
direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
Looking for similar products? Visit Product Comparison Guide
Application
Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:
- western blotting
- immunohistochemistry
- flow cytometry
- direct immunofluorescent staining
- dual immuno-fluorescent staining
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Biochem/physiol Actions
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.
Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.
Immunogen
slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Rajeevkumar R Nair et al.
Frontiers in synaptic neuroscience, 9, 8-8 (2017-05-30)
Activity-regulatedcytoskeleton-associated protein (Arc) protein is implicated as a master regulator of long-term forms of synaptic plasticity and memory formation, but the mechanisms controlling Arc protein function are little known. Post-translation modification by small ubiquitin-like modifier (SUMO) proteins has emerged as
Decreased expression of angiogenesis antagonist EFEMP1 in sporadic breast cancer is caused by aberrant promoter methylation and points to an impact of EFEMP1 as molecular biomarker
Sadr NA, et al.
International Journal of Cancer. Journal International Du Cancer, 124(7), 1727-1735 (2009)
CD9 regulates transcription factor GCM1 and ERVWE1 expression through the cAMP/protein kinase A signaling pathway
Muroi Y, et al.
Reproduction (Cambridge, England), 138(6), 945-951 (2009)
Zurab Siprashvili et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 9(5), 721-728 (2004-05-04)
Despite numerous attractive intracellular targets, protein therapeutics have been principally confined to the extracellular space due to the lack of a straightforward way to deliver functional polypeptides to the cell interior. Peptide sequences facilitating intracellular protein delivery have been identified;
Shuan Rao et al.
Nature communications, 5, 3056-3056 (2014-01-22)
Autophagy is a mechanism by which starving cells can control their energy requirements and metabolic states, thus facilitating the survival of cells in stressful environments, in particular in the pathogenesis of cancer. Here we report that tissue-specific inactivation of Atg5
Related Content
Instructions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service