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Merck
CN

F3543

Sigma-Aldrich

Fluorescent Brightener 28

used as a stain and brightening agent

Synonym(s):

Calcofluor White M2R, Tinopal UNPA-GX

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About This Item

Empirical Formula (Hill Notation):
C40H44N12O10S2
CAS Number:
4404-43-7
Molecular Weight:
916.98
EC Number:
224-548-7
MDL number:
MFCD00308955
UNSPSC Code:
12171500
PubChem Substance ID:
24894849
NACRES:
NA.47

Key Documents

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form

powder

mp

300 °C

εmax

40-60 at 238-242 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S(O)(=O)=O)c(c3)S(O)(=O)=O)n1

InChI

1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+

InChI key

CNGYZEMWVAWWOB-VAWYXSNFSA-N

General description

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
Fluorescent Brightener 28 has been used for the staining of fungal cell walls. It has also been used for the staining of Candida albicans biofilm.

Application

It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCJ1547
MKCG0733
MKCD8752
MKCB3546
SLBM3344V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How is Product F3543, Fluorescent Brightner 28, dissolved for use?

    The product can be diluted in DMSO at a concentration of 50 mg/ml.  The product can be dissolved in water at a concentration of 1 mg/ml, although the use of 50% sodium hydroxide might be required to fully solubilize the product.

  4. What are the excitation and emission wavelengths for Product F3543, Fluorescent Brightner 28?

    Excitation is at 365 nm and emission at is at 435 nm.

  5. Is there a reference for use of Product F3543, Fluorescent Brightner 28?

    3 different staining applications are found in the following reference:  Clark, G; Staining Procedures, 4th Edition, Williams & Wilkins, Baltimore 1981 pages 46-48. This product is used to monitor removal or regeneration of plant cell walls. A protocol for using this product with plant protoplasts is described. D.W. Galbraith, Physiologia Plantarum, 53, 111-116 (1981). 

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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    Ask a Scientist here.

Joshua Edwards et al.
The Plant journal : for cell and molecular biology, 63(4), 651-661 (2010-06-16)
Transfer cells are specialised transport cells containing invaginated wall ingrowths that generate an amplified plasma membrane surface area with high densities of transporter proteins. They trans-differentiate from differentiated cells at sites at which enhanced rates of nutrient transport occur across
Christine Seah et al.
Medical mycology, 50(3), 324-327 (2011-08-24)
We compared the FXG™: RESP (Asp +) real-time PCR assay (Myconostica Ltd) with two microscopic staining methods (direct immunofluorescence [IFA] and calcofluor white) for the detection of Pneumocystis jirovecii in 411 respiratory specimens submitted for P. jirovecii examination. We considered
Luis Vidali et al.
PloS one, 4(5), e5744-e5744 (2009-05-30)
Actin is essential for tip growth in plants. However, imaging actin in live plant cells has heretofore presented challenges. In previous studies, fluorescent probes derived from actin-binding proteins often alter growth, cause actin bundling and fail to resolve actin microfilaments.
Candida albicans biofilm-defective mutants.
Richard ML
Eukaryotic Cell, 4, 1493-1493 (2005)
Rumsaïs Blatrix et al.
Proceedings. Biological sciences, 279(1744), 3940-3947 (2012-08-04)
Usually studied as pairwise interactions, mutualisms often involve networks of interacting species. Numerous tropical arboreal ants are specialist inhabitants of myrmecophytes (plants bearing domatia, i.e. hollow structures specialized to host ants) and are thought to rely almost exclusively on resources
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