Key Documents

View All Documentation

F3651

Fluorescein 5(6)-isothiocyanate

Name: Fluorescein 5(6)-isothiocyanate
Brand: SIGMA

≥90% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein isothiocyanate

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₁NO₅S

CAS Number:

27072-45-3

Molecular Weight:

389.38

NACRES:

NA.47

PubChem Substance ID:

24894855

UNSPSC Code:

12171500

EC Number:

248-207-7

MDL number:

MFCD00005063

Beilstein/REAXYS Number:

1407295

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Product Name

Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)

Quality Level

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein 5(6)-isothiocyanate has been used:

for fluorescence labeling of bacteria
as a standard for calibration of fluorometer
for the labeling of exosomes

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Storage Class

11 - Combustible Solids

wgk

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000509992

0000494087

0000433649

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Effects of supplemental 25-hydroxycholecalciferol on growth performance, small intestinal morphology, and immune response of broiler chickens.

Chou SH

Poultry Science, 88, 2333-2333 (2009)

Characterization of proteolytic enzyme activities in macroalgae.

Perez-Llorens JL

European Journal of Phycology, 38, 55-64 (2003)

Thymic exosomes promote the final maturation of thymocytes.

Lundberg V

Scientific Reports, 6, 36479-36479 (2016)

Hermanson GT

Bioconjugate Techniques (2013)

Handbook of Affinity Chromatography (2005)

View All Related Papers

Global Trade Item Number

SKU	GTIN
F3651-100MG	04061833615218

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service