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Merck
CN

F4648

Fast Red TR/Naphthol AS-MX

SIGMAFAST™, fluorogenic, tablet

Synonym(s):

4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
MDL number:
MFCD00132253

Key Documents

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Product Name

SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL

form

tablet

solubility

water: 1 tablet/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

Quality Level

200

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Application

Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
  • in alkaline phosphatase staining in organ sections
  • for staining chondrogenic spheroids in immunohistochemistry studies
  • for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections

Preparation Note

Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

hcodes

H314,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000419765
0000419765
0000419761
0000419761
SLCL4119

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Tony Y-C Tsai et al.
Science (New York, N.Y.), 370(6512), 113-116 (2020-10-03)
Animal development entails the organization of specific cell types in space and time, and spatial patterns must form in a robust manner. In the zebrafish spinal cord, neural progenitors form stereotypic patterns despite noisy morphogen signaling and large-scale cellular rearrangements
Anja Rabien et al.
International journal of molecular sciences, 19(6) (2018-06-03)
Bladder cancer still requires improvements in diagnosis and prognosis, because many of the cases will recur and/or metastasize with bad outcomes. Despite ongoing research on bladder biomarkers, the clinicopathological impact and diagnostic function of miRNA maturation regulators Drosha and Argonaute
Kathryn B Moore et al.
Developmental biology, 437(1), 27-40 (2018-03-09)
Neural basic helix-loop helix (bHLH) transcription factors promote progenitor cell differentiation by activation of downstream target genes that coordinate neuronal differentiation. Here we characterize a neural bHLH target gene in Xenopus laevis, vexin (vxn; previously sbt1), that is homologous to
Jason S Williams et al.
Developmental biology, 444 Suppl 1, S274-S286 (2018-04-01)
Melanocytes derive from neural crest cells, which are a highly migratory population of cells that play an important role in pigmentation of the skin and epidermal appendages. In most vertebrates, melanocyte precursor cells migrate solely along the dorsolateral pathway to
PTD-mediated loading of tumor-seeking lymphocytes with prodrug-activating enzymes
Yang Q, et al.
The AAPS Journal, 10(4), 614-614 (2008)
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