F5554
Formalin, 10%, Neutral Buffered, Wintergreen Scented
histological fixative, pH 6.9-7.1
Synonym(s):
Formaldehyde, Methanal
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About This Item
NACRES:
NA.47
UNSPSC Code:
12171500
Quality Level
shelf life
Expiry date on the label.
color
colorless
pH
6.90-7.10
application(s)
hematology
histology
storage temp.
room temp
General description
10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.
Application
Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)
Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
Magdeldin, et al.
Proteomics, 12(7), 1045-1058 (2012)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)
Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung
Donnenberg VS, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 83(1), 141-149 (2013)
Kelly B Engel et al.
Archives of pathology & laboratory medicine, 135(5), 537-543 (2011-04-30)
While formalin fixation and paraffin embedding has become a universal mechanism of tissue preservation and a gold standard for immunohistochemistry, fixation and processing variables that may confound assay effectiveness have received little attention from the scientific community. To identify discrete
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F5554-4L | 04061838207104 |
| F5554-19L | 04061838207098 |
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