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F6140

Anti-Fibronectin Antibody

Name: Anti-Fibronectin Antibody
Brand: SIGMA

mouse monoclonal, FN-3E2

Synonym(s):

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164562

Conjugate:

unconjugated

Clone:

FN-3E2, monoclonal

Application:

IF, IHC (f), IHC (p), IP, RIA, WB

Citations:

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Product Name

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse, clone FN-3E2, ascites fluid

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FN-3E2, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

mouse, human, chicken

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect immunofluorescence: 1:400 using cultured chicken fibroblasts, radioimmunoassay: suitable, western blot: suitable

isotype

IgM

UniProt accession no.

P02751

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

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Related Categories

Antibodies - Primary, Secondary & Recombinant Monoclonal

Antibodies by Application

Primary Antibodies

General description

Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.

Immunogen

fibronectin antigen released in culture by a human breast cancer cell line

Application

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:

immunoblot
immunofluorescent microscopy
immunocytochemistry

Biochem/physiol Actions

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .

Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

动物来源生物产品

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Fibronectin in cell adhesion and migration via N-glycosylation

Hsiao CT, et al.

Oncotarget, 8(41), 70653-70653 (2017)

Texas 3-step decellularization protocol: looking at the cardiac extracellular matrix.

Lisandra E de Castro Brás et al.

Journal of proteomics, 86, 43-52 (2013-05-18)

The extracellular matrix (ECM) is a critical tissue component, providing structural support as well as important regulatory signaling cues to govern cellular growth, metabolism, and differentiation. The study of ECM proteins, however, is hampered by the low solubility of ECM

Fibronectin (cold-insoluble globulin): role in defence.

A Vaheri

Schweizerische medizinische Wochenschrift, 110(40), 1437-1440 (1980-10-04)

Fibronectin is a high molecular weight glycoprotein which is found in a soluble form in plasma (cold-insoluble globulin) and other body fluids and in an insoluble form in connective tissues and associated with basement membranes. Soluble fibronectin interacts with denatured

Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment

McLane JS, et sl.

Biophysical Journal, 109 (2015)

ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions

Cain SA, et al.

Scientific reports, 6 (2016)

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Global Trade Item Number

SKU	GTIN
F6140-100UL	04061837883675
F6140-.2ML	04061837883668

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