F7053
Anti-Vinculin−FITC antibody, Mouse monoclonal
clone hVIN-1, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Vinculin antibody produced in mouse
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About This Item
Conjugate:
FITC conjugate
Clone:
hVIN-1, monoclonal
Application:
direct immunofluorescence
Species reactivity:
frog, chicken, mouse, canine, human, bovine, rat, turkey
Citations:
18
Technique(s):
direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells
Uniprot accession no.:
Product Name
Anti-Vinculin−FITC antibody, Mouse monoclonal, clone hVIN-1, purified from hybridoma cell culture
biological source
mouse
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hVIN-1, monoclonal
form
buffered aqueous solution
mol wt
antigen 116 kDa
species reactivity
frog, chicken, mouse, canine, human, bovine, rat, turkey
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
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Application
Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.
Biochem/physiol Actions
Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.
Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
purified human vinculin from uterus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Akiko Hashimoto-Tane et al.
The Journal of experimental medicine, 213(8), 1609-1625 (2016-06-30)
The immunological synapse (IS) formed at the interface between T cells and antigen-presenting cells represents a hallmark of initiation of acquired immunity. T cell activation is initiated at T cell receptor (TCR) microclusters (MCs), in which TCRs and signaling molecules
Mathieu Maalouf et al.
Nanomaterials (Basel, Switzerland), 12(10) (2022-05-29)
Ultrashort pulse lasers have significant advantages over conventional continuous wave and long pulse lasers for the texturing of metallic surfaces, especially for nanoscale surface structure patterning. Furthermore, ultrafast laser beam polarization allows for the precise control of the spatial alignment
Viorel-Iulian Suica et al.
Proteome science, 13, 30-30 (2015-12-03)
Membrane microdomains represent dynamic membrane nano-assemblies enriched in signaling molecules suggesting their active involvement in not only physiological but also pathological molecular processes. The hyperlipidemic stress is a major risk factor of atherosclerosis, but its exact mechanisms of action at
Subhashree Shivani et al.
Frontiers in cell and developmental biology, 10, 825791-825791 (2022-04-09)
The mediation of the extracellular matrix is one of the major environmental cues to direct cell migration, such as stiffness-dependent durotaxis and adhesiveness-dependent haptotaxis. In this study, we explore another possible contact guidance: roughness dependent topotaxis. Different from previously reported
S Hüttelmaier et al.
Current biology : CB, 8(9), 479-488 (1998-05-20)
Focal adhesion sites are cell-matrix contacts that are regulated by phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent pathways. Vinculin is a major structural component of these sites and is thought to be engaged in multiple ligand interactions at the cytoplasmic face of these contacts. Cytoplasmic
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