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F7253

Fast Black K Salt hemi(zinc chloride) salt

Name: Fast Black K Salt hemi(zinc chloride) salt
Brand: SIGMA

Dye content, ≥25%, practical grade, powder

Synonym(s):

2,5-Dimethoxy-4-([4-nitrophenyl]azo)benzenediazonium chloride hemi(zinc chloride) salt, Brentamine fast black K salt

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About This Item

Linear Formula:

C₁₄H₁₂N₅O₄ · 1/2ZnCl₄

CAS Number:

64071-86-9

Molecular Weight:

417.88

Colour Index Number:

37190

UNSPSC Code:

12171500

PubChem Substance ID:

24894935

EC Number:

264-656-1

NACRES:

NA.47

MDL number:

MFCD00078471

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Properties

Product Name

Fast Black K Salt hemi(zinc chloride) salt, practical grade

grade

practical grade

Quality Level

200

form

powder

composition

Dye content, ≥25%

technique(s)

microbe id | staining: suitable

color

brown-red

mp

150 °C

solubility

water: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1N=Nc2ccc(cc2)[N+]([O-])=O.COc3cc([N+]#N)c(OC)cc3N=Nc4ccc(cc4)[N+]([O-])=O

InChI

1S/2C14H12N5O4.4ClH.Zn/c2*1-22-13-8-12(14(23-2)7-11(13)16-15)18-17-9-3-5-10(6-4-9)19(20)21;;;;;/h2*3-8H,1-2H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

NZWZSUSPTZUGNN-UHFFFAOYSA-J

Description

General description

Fast black K (C.l. 37190) is a lipophilic amino-substituted monoazo dye. It is also known as Brentamine fast black K salt.

Application

Fast Black K Salt hemi (zinc chloride) salt has been used for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE. It has been used for staining endopeptidase and leucine aminopeptidase activity.

Fast black K salt has various applications as listed below-

to stain enterochromaffin cells > as an indirect stain for periodate-generated tissue aldehyde groups
as a visualization agent for amphetamines and cyanobacterial toxins following their separation by thin-layer chromatography
for the staining of acid phosphatase activity in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and native-PAGE
staining endopeptidase and leucine aminopeptidase activity

pictograms

GHS07

signalword

Warning

hcodes

H319

pcodes

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Genetic control of isozymes in the primary gene pool Phaseolus lunatus L.

Zoro BI

Biotechnologie, agronomie, societe et environnement, 3, 10-27 (1999)

Analyses of Root-secreted Acid Phosphatase Activity in Arabidopsis.

Wang L and Liu D

Bio-protocol null

Global Trade Item Number

SKU	GTIN
F7253-25G	04061835347711
F7253-50G	04061833619414
F7253-100G	04061835347704