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F8687

Sigma-Aldrich

Fetal Bovine Serum

Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture, suitable for hybridoma

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Synonym(s):
Fetal Bovine Serum, FBS, Fetal Bovine Sera, GI FBS, GI FCS, GI Fetal Bovine Sera, GI Fetal Bovine Serum, FCS, sera, serum
MDL number:
MFCD00132239

biological source

bovine fetus

Quality Level

500

sterility

γ-irradiated
sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤0.18 mg/mL

origin

Australia origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤5 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used in the Roswell Park memorial institute medium-1640 (RPMI-1640) medium to culture myeloma cell lines, RPMI-8226 and U-266. It has also been used as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture human umbilical vein endothelial cells (HUVECs) and 293 T cells.

Biochem/physiol Actions

Fetal Bovine Serum (FBS) is the commonly used serum to supplement cell culture media due to its high nutritional content. FBS, despite its low protein content, is effective in promoting and maintaining the growth of vertebrate mammalian and insect cells. It can be used as a supplement to culture embryonic stem (ES) cells. FBS can also be used to prepare feeder layers and conditioned media.

Linkage

Learn more information of serum

Analysis Note

  • Endotoxin and hemoglobin tested

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Qingqing Wang et al.
Biochemical and biophysical research communications, 549, 1-7 (2021-03-02)
Ixazomib is a selective, effective, and reversible inhibitor of 20S proteasome and is approved for the treatment of multiple myeloma. Ubiquitin-conjugating enzyme E2 (UBE2K) is involved in the synthesis of K48-linked ubiquitin chains and is the target of certain drugs
Peng Zhang et al.
International journal of stem cells, 14(2), 229-239 (2021-02-27)
Implantation of bone marrow-derived mesenchymal stem cells (BMSCs) has been recognized as an effective therapy for attenuating acute lung injury (ALI). This study aims to discover microRNA (miRNA)-mediated improvement of BMSCs-based therapeutic effects. Mice were treated with lipopolysaccharide (LPS) for
Yan Zhang et al.
Artificial cells, nanomedicine, and biotechnology, 48(1), 60-67 (2019-12-20)
Background: Colon cancer is the most frequently lethal cancer in digestive system. Herein, we tested the influences of UNC5B antisense lncRNA 1 (UNC5B-AS1) on colon cancer cell growth and metastasis, along with the regulatory function of UNC5B-AS1 in microRNA-622 (miR-622)
Isolation and maintenance of avian ES cells
Petitte JN
Handbook of Stem Cells, 471-477 (2004)
Baculoviruses: Expression Vector
Haines FJ, et al.
Encyclopedia of Virology (2008)
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Articles

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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