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About This Item
MDL number:
UNSPSC Code:
12352207
Biological source:
bovine fetus
Origin:
Australia origin
Sterility:
γ-irradiated
sterile-filtered
sterile-filtered
Shipped in:
dry ice
biological source
bovine fetus
Quality Level
sterility
γ-irradiated
sterile-filtered
product line
FBS Classic
composition
hemoglobin, ≤0.18 mg/mL
origin
Australia origin
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
impurities
≤5 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
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Application
Fetal Bovine Serum has been used in the Roswell Park memorial institute medium-1640 (RPMI-1640) medium to culture myeloma cell lines, RPMI-8226 and U-266. It has also been used as a supplement in Dulbecco′s modified Eagle medium (DMEM) to culture human umbilical vein endothelial cells (HUVECs) and 293 T cells.
Biochem/physiol Actions
Fetal Bovine Serum (FBS) is the commonly used serum to supplement cell culture media due to its high nutritional content. FBS, despite its low protein content, is effective in promoting and maintaining the growth of vertebrate mammalian and insect cells. It can be used as a supplement to culture embryonic stem (ES) cells. FBS can also be used to prepare feeder layers and conditioned media.
Analysis Note
- Endotoxin and hemoglobin tested
Other Notes
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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