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F8799

Fas/Fc Chimera from mouse

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

Apo-1, CD95

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
MFCD01867037

Key Documents

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Product Name

Fas/Fc Chimera from mouse, >97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

recombinant

expressed in baculovirus infected Sf21 cells

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)

mol wt

55 kDa (glycosylated form)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P25446

storage temp.

−20°C

Quality Level

200

Gene Information

mouse ... Fas(14102)

Analysis Note

Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.

Application

Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).

Biochem/physiol Actions

Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).

General description

Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
091M1506
081M1218
061M1220
129K2178
010M1089

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Davidson D Ateh et al.
Biomaterials, 32(33), 8538-8547 (2011-08-10)
The CD95/CD95L receptor-ligand system is mainly recognised in the induction of apoptosis. However, it has also been shown that CD95L is over-expressed in many cancer types where it modulates immune-evasion and together with its receptor CD95 promotes tumour growth. Here
Cheng Jin et al.
Medical science monitor : international medical journal of experimental and clinical research, 21, 630-637 (2015-02-28)
The CD95 gene plays a key role in regulating cell growth and tumor genesis. To date, several publications have focused on the CD95 rs1800682A/G site polymorphism and various types of tumors in Asians; however, this association is still controversial and
J Cheng et al.
Science (New York, N.Y.), 263(5154), 1759-1762 (1994-03-25)
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because
B A Treviño-Talavera et al.
Genetics and molecular research : GMR, 13(3), 4831-4838 (2014-07-26)
Primary Sjögren's syndrome (pSS) is a chronic systemic autoimmune disease characterized by lymphocytic infiltration of exocrine glands. Soluble Fas receptor (sFas) has been suggested as a Fas-mediated apoptosis blocker that could impair clonal deletion in infiltrated autoreactive cells. The FAS
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
View All Related Papers

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Data Sheet - F8799

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