ISF-1 Medium

Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies.

This product is identical to Biochrom GmbH part number F9061-01. ISF-1 is designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation.

This proprietary formulation includes inorganic salts, essential and non-essential amino acids, stable glutamine, vitamins, sodium bicarbonate, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG).

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8 °C in the dark, medium that has not been supplemented is stable until the expiration date on the label.